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Abstract
There are some fatal diseases facing newly born calves specially
those of viral nature. Two major causative viral agents of these diseases are bovine coronavirus (BCV) and bovine rotavirus (BRV) which are
most familiar causes of neonatal calf diarrhea. Detection of these viruses
in affected animals in addition to monitoring of the immune status of
calves against such viruses requires the presence of available, effective
Kits of good quality and low cost. So the present work was planned to
prepare diagnostic Kits for BCV and BRV in trials to provide local
products usually in essential need .These locally prepared diagnostic Kits are saving time and high cost which could be needed to obtain similar
imported products.
The obtained experimental results revealed that: 1- The most suitable susceptible cell cultures for BCV and BRV were MA104 and MDBK yielding virus titers of7.5 and 8 10glO TC1Dso/ml for
BCV and BRV, respectively. VERO cells were found to be less
susceptible for both BCV and BRV showing virus titers of 6 and 6.5 10glO
TCIDso/ml, respectively. While, BHK cells were found unsusceptible for
propagation of both viruses.
2-It was successful to prepare anti-BCV and anti-BRV hyperimmune sera
in rabbits of good quality as shown by SNT, AGPT and estimation of
serum protein which revealed that the immunoglobulin contents were 6.4 . gldl and 10.5 gldl for BCV and BRV-antisera, respectively.
3-Chekerboard ELISA indicated that the locally prepared whole cell and
cell lysate antigens (CLA) of bovine BCV and BRV had optimal dilutions
of (1/30 &1150) and (1/40 &1150), respectively.