الفهرس 
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Abstract
Total and absolute white blood cell count
The present data showed that fractionated doses of whole body gamma-irradiation (1Gy/3times) of pregnant rats resulted in significant decrease in the total white blood cell count and absolute lymphocyte count as well as absolute neutrophil count of pregnant rats. Fractionated doses of gamma -irradiation caused monocytopenia in pregnant rats.
Green tea administration at two concentrations 1.5% and 3% did not change significantly total white blood cell count and absolute lymphocyte and neutrophil counts as compared with control one. But in case of absolute monocyte count showed significant decrease in group Gt., 1.5%.
Green tea administration at two concentrations concurrent with y- irradiation showed amelioration effects in lymphocyte and white blood cell counts in Gt., 1.5%+Ir. and Gt., 3%+Ir. groups. Meanwhile Green tea administration in the two concentrations 1.5% and 3% concurrent with g-rays exposure ameliorated significantly absolute neutrophil count in group Gt., 1.5%+Ir. and did not ameliorate absolute neutrophil count in group Gt., 3 %+Ir.
Green tea administration concurrent with y- irradiation did not improve the reduction in monocyte count in group Gt., 1.5%+Ir. It improved the reduction in absolute monocyte count in group Gt., 3%+Ir.
Red blood cells count, haemoglobin content, haematocrit value and blood indices
In the present investigation, the data obtained showed that fractionated doses of whole body gamma- irradiation induced decrease in red blood cells count, haemoglobin content and haematocrit value in pregnant rats. Meanwhile, mean cell haemoglobin concentration was decreased significantly, mean cell volume was increased significantly and the mean cell haemoglobin did not change.
Oral administration of green tea extracts at two concentrations 1.5% and 3% did not change significantly the number of erythrocytes, haematocrit value, haemoglobin content and blood indices as compared with the control, except group Gt.,3% showed significant increase in red blood cells count
Green tea administrated to group Gt., 1.5% and group Gt., 3% from the 1st day rill the 20th day gestation for 5 times / week concurrent with irradiation with 1Gy/3 times at the 7th, 11th and 15th days of pregnancy showed improvement in red blood cells count and haemoglobin content as well as haematocrit value.
Serum glucose concentration
The present results indicated that fractionated doses of whole body gamma -rays exposure with 1Gy/3 times caused increase in serum glucose concentration. Green tea administrated group Gt.,3% showed
significant increase in serum glucose concentration. Gt., 1.5% group showed non-significant increase in serum glucose concentration as compared with control group. On the other hand, green tea administration concurrent with 7-rays exposure showed significant reversed of the increase in serum glucose concentration in group Gt., 1.5%+ft. and group Gt., 3%+Ir.
Serum total protein concentration
In the present study, the data obtained showed that fractionated doses of whole body gamma- irradiation decreased serum total protein concentration.
Oral green tea infusion in the two concentrations 1.5% and 3% to pregnant rats without exposure to gamma-rays showed non-significant changes in total protein concentration in group Gt., 1.5 % and Gt.,3% group as compared with control group.
On the other hand, pregnant rats administrated with green tea at 1.5% and 3% concentrations before and during fractionated doses of gamma-rays, exhibit pronounced amelioration effect on the radiation-induced decrease in serum total protein concentration.
Serum urea, uric acid and creatinine concentrations
Current results showed pronounced increase in serum urea, uric acid and creatinine concentrations after whole body gamma-rays exposure at fractionated doses of 1Gy/ 3 times.
Blood lipid peroxidation
In the present Study, significant increase in serum level of the malondialdehyde (as indicator to level of lipid peroxidation) was detected in pregnant rats at the 20th of pregnancy after exposure to fractionated doses of whole body gamma- irradiation.
Oral infusion of green tea at two concentrations 1.5 % and 3% to pregnant rats without exposure to gamma-radiation showed non-significant change in lipid peroxidation level compared with control one.
On the other hand, rats were administrated green tea before and during irradiation exhibited a pronounced protective effect on the radiation-induced increase in lipid peroxidation of group Gt., 3%+Ir. and group Gt., 1.5% +Ir.
Blood glutathione content (reduced form)
The present observation showed a highly significant increase in blood level of glutathione content of pregnant rats after exposure to fractionated doses of whole body gamma- rays.
Green tea administration at two concentrations 1.5% and 3% without exposure to gamma- rays did not change significantly blood glutathione content as compared with control group, whereas green tea administration to irradiated group showed amelioration effect on the increase of blood glutathione content in group Gt.,1.5%+Ir. and Gt., 3%+Ir. group.
meanwhile Gt., 3% group showed pronounced increase in AST enzyme activity.
Green tea administration at the concentrations 1.5% and 3% concurrent with gamma-irradiation depressed the increase in serum ALT enzyme activity of group Gt.,3%+Ir. and group Gt.,1.5%+Ir. as well as improved the decrease in serum AST enzyme activity in group Gt.,3%+Ir. and did not improve the decrease in AST of group Gt.,1.5%+Ir.
Teratological observations
The results indicated that fractionated doses (1Gy/3times) of whole body gamma-irradiation resulted in abnormal features, some uteri exhibited implantation sites only as an evidence of abortion. Prenatal mortality and shortness of one horn, besides unequal distribution of the fetuses in the two horns were observed. The number of fetuses in each animal ranged from 3 to 5 embryos. Size and length of fetuses were lowed than control one.
In group Gt., 1.5% and group Gt.,3% , abnormal features were not observed Implantation sites, prenatal moralities were not noticed, also equal distribution of fetuses in the two horns of uteri. The number of fetuses in some animals is ranged from 6 to 9 embryos or may be increase in other animals.
3-Intestine
The present result indicated that fractionated doses of gamma- rays exposure (1 Gy / 3 times) resulted in absence of the villous pattern. The crypts are dilated and the mucosa shows severe chronic
inflammation.
In the present study, the effect of green tea orally administration at two concentrations 1.5 % and 3% on the intestine without gamma- rays exposure showed congestion of the wall and regeneration of basal cells in group Gt., 1.5 % and crypt dilation and neutrophilic infiltration of the submucosa in Gt., 3% group .
The effect of green tea infusion at the two concentrations 1.5% and 3% before and during exposure to fractionated 3 Gy 7- rays is indicated by epithelial cell repair. Restoration of normal epithelial pattern and cellular integrity were roughly detected besides division of nuclei in slight regenerative attempts. However, still, enlarged villi and severe chronic inflammation, several villi tips appeared to be fusion into a large villus, but remaining separated at the base in Gt., 1.5% +Ir. Group. Ramifications of crypt, enlarged and shortened villi and clear spaces also were seen in the lamina propria located in the basal part of the villi in Gt., 3%+Ir. Group.