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Abstract
A trial to investigate the efficacy of different propolis preparations ( BEE
Glue) as anti bacterial and anti fungal natural remedy has been carried out
chickens. Some in vitro and in vivo experiments were carried out to evaluate its efficacy against E. coli, S. typhimurium . St .aureus and A. flavus. In vitro studies of purified, Ethanolic (EEP) and aqueous (APE) extracts of propolis revealed that, purified propolis had inhibitory effects against E. coli but not against A. flavus, the disc sensitivity test was the best in its evaluation with an inhibitory zone of 15 mm . Investigation ofEEP revealed that 50 mg was effective only against E coli
while 100 & 200 mg were needed for inhibition of ( E. coli ” S.
typhimurium. Staph .aureus and A. flavus. APE showed no inhibitory effect
except against S. typhimurium .
Different propolis preparations were investigated against both E. coli and
flavus In vivo . The results revealed that a dose of 100 mg / kg BW
reduced the clinical signs, mortality rate and PM lesions as well as A. flavus shedding. The best results were recorded in the birds supplemented with
propolis in ration which revealed reduction of clinical signs to 20 % in
comparison to non treated of 80 % . Moreover No fungal reisolation was
recorded post treatment .
Administration via DW and IM showed less protection. However, there
was an improvement in body weight gain in all treated groups in comparison
I ( with infected non treated.
Intra tracheal application of 108CFU / 0.1 ml E . coli was the route of
choice and IM injection of propolis had suitable for treatment application in