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Abstract
Artificial hatching is a method by which the eggs that otherwise would naturally undergo diapause are treated to make them hatch artificially during the course of the year.
The present study was therefore undertaken to study the effect of different methods of artificial hatching of B. mori L. eggs to be reared during two rearing seasons; early and late Autumn seasons on the hatching performance, the biological parameters, the most important economical characters as well as the 5th larval instar haemolymph component and separation of non-specific esterase activity.
Larvae of three races of B. mori L. two Japanese races (J2MJ and J2PJ) and one Chinese race Dch were used and five different methods of artificial hatching were selected according to the next desired rearing season and applied to the obtained eggs from Spring rearing season:
1. Two artificial hatching methods after cold storage with a special regime for rearing early Autumn for Spring egg laying by using
a. Hot acid treatment after cold storage
Egg-cards were dipped in 2% formaldehyde solution for 2-10 minutes to make the egg adhere firmly to the egg-cards, egg cards were dipped in Hcl with specific gravity 1.160 for ten seconds to discard the moisture, then dipped in hot acid solution at 47.8 C° at the same specific gravity for 5.5-6 minutes for Japanese races and 5-5.5 minutes for Chinese race then washed in running water and dried. Egg- cards were incubated at 24-26 C° and 80-90% R.H. till hatching (TA).
b. Hot water treatment after cold storage Egg-cards were dipped at hot water of 48C° for 10 seconds then immersed in formaldehyde solution 2% for 30 minutes then washed and dried. Treated eggs were incubated at 24-26 C° and 80-90 %R.H. until hatching (TB).