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Abstract This work was conducted in Faculty of Veterinary Medicine, Physiology department, where it spotted on the effect of some environmental and physiological factors such as temperature, photoperiod and pheromones in regu lating gonadal development in both sexes. The study was conducted on 260 fish of Tilapia nilotica during the period from March to July. The fish were divided into 3 groups, where 80 fish for each temperature & photoperiod experiment and 1000 for the pheromone experiment. Male & female parameter such as GSI, sperm count, motility, fecundity’, oocyte maturation were measured. Moreover, sex steroid hormones E2 & T and calcium level in both sexes. Result showed that temperature of 27 & 30 after 20 days has beneficial effect on the maturity of both sexes, modulation of photoperiod regimes. were non- significant, and rearing males with females in the same aquarium . |