![]() | Only 14 pages are availabe for public view |
Abstract The irradiated and unirradiated samples of twelve amino acids at 0.01 and 0.05 molar concentration were analysed using one dimension parper chromatography. Colour densities and spot areas of the amino acids revealed that the amount of all amino acids were reduced by increasing the radiation doses. Also, the ninhydrin positive products resulted from the degradation of the irradiated amino acids increased in concentration by increasing radiation doses. Quantitative determination proved that all amino acids studied at both 0.01 and 0.05 m concentration were destroyed but at different degrees. In case of 0.01 m concentration there was a linear relation between the percentage of remaining amounts of amino acid and the radiation doses. The order of amino acids sensitivity to gamma radiation at both concentration was as follows :phenylalanine >methionine> arginine> serine >isoleucine> leucine> glutamic acid>lysine-hcl>tryptophan>B-alanine>glycine>tyrosine |