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Abstract Whe bactericidal activity of phenol against StaphoCOCCUS aureu and. Psudomonas aeruginosa could be enhanced with 0,06% Tween. 80, while higher concentrations reduced the activity of the bactericide. The improved activity could be due to the effect of Tween on the cell surf ace, since the death rates of phenol on Tween—treated bacteria were double those of phenol on untreated bacteria. The results also indicated that Tween 80 exerted certain suble— thai action on bacteria rendering then more sensitive to the action of phenol. However, all the phenol—Tween 80 combinations were of lower bactericidal activities than the activity exhibited by phenol on Tween—treated. cells. This difference was attributed to the interaction of phenol with theTween 80. The extent of interaction depended upon the concentration of the non—ionic. |