الفهرس 
Introduction and Aim of the WorkOnly 14 pages are availabe for public view
 |  | from | 106 |  |  |
| | | from | 106 | | |
Abstract
Aim of the work
1- To evaluate the reliability of ELISA technique and glutaraldehyde test in diagnosis of pulmonary tuberculosis.
2- Study the association of HLA and children with pulmonary tuberculosis.
On conclusion, because the diagnosis of tuberculosis in children in developing countries is still difficult due to scanty laboratory facilities that depend on sputum with positive mycobacterial bacilli which is very difficult to be obtained especially in primary pulmonary tuberculosis so, using indirect methods for diagnosis of might be helpful as chest x-ray and tuberculin skin test can support the diagnosis of T.B. as they were positive in 84.2% and 81.4% respectively in the studied cases of pulmonary tuberculosis.
Concerning the Glutaraldehyde (Gelification) test, its results showed high sensitivity and specificity they were 87.1% and 90% respectively with high significance (p<0.001) the test was simple, rapid, inexpensive and did not need highly trained personnels in addition to it was not tedious and was positive in cases with malnutrition (P.E.M) so, it can be used as an adjuvant diagnostic tool for tuberculosis.
Concerning ELISA test using A60 antigen, its use showed that the sensitivity of the test was 48.6% whereas specificity was 90% with high significance (p<0.01) so the test can be used as a rapid serodiagnostic test which is reliable and relatively in expensive technique for diagnosis of active pulmonary T.B. in children. Also, from our study we have concluded that combining ELISA, Glutaraldehyde and tuberculin tests together with the clinical and radiological findings provide the best chance for diagnosis of pulmonary tuberculosis in children and replace the bacteriologic method of diagnosis of which is not very helpful especially in children with primary pulmonary tuberculosis.