الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Organic nitrates are among the oldest and most commonly used drugs for the treatment of cardiovascular diseases. When nitrates are metabolized to release NO, there is a considerable coproduction of reactive oxygen species as superoxide radical and reactive nitrogen species as peroxynitrite these free radicals may increase oxidative stress and lipid peroxidation which can cause many harmful side effects.
Forty two male wistar rats weighing about 150-180 g were used in the present study. The experimental animals were divided into seven main groups: A) Group (1) control group:Rats were received normal laboratory diet without any treatment. B) Group (2):
Rats of this group were supplied with normal laboratory diet and received orally ISMN with a gastric tube at a dose of 1.7 mg/ kg body weight every day for a period of 2 months. C) Group (3) Rats of this group were supplied with normal laboratory diet and received orally ISMN at the same dose of group (2) followed by vitamin C at a dose of 28.5 mg/ kg body weight for the same period 2 months.D) Group (4): Rats of this group were supplied with normal laboratory diet and received orally ISMN with a gastric tube at a dose of 3.4 mg/ kg body weight every day for a period of 2 months. E) Group (5) Rats of this group were supplied with normal laboratory diet and received orally ISMN at the same dose of group (4) followed by vitamin C at a dose of 28.5 mg/ kg body weight for the same period 2 months. F) Group (6): Rats of this group were supplied with normal laboratory diet and received orally ISMN with a gastric tube at a dose of 6.8 mg/ kg body weight every day for a period of 2 months.
g) Group (7) Rats of this group were supplied with normal laboratory diet and received orally ISMN at the same dose of group (6) followed by vitamin C at a dose of 28.5 mg/ kg body weight for the same period 2 months.
At the end of the experimental period 2 months, blood samples were collected in clean centrifuge tubes for biochemical investigations of total hemoglobin, methemoglobin, carboxyhemoglobin, saturated oxygen, oxygen content, hematocrit, red blood cells, white blood cells, nitric oxide, glutathione, catalase, superoxide dismutase, malondialdehyde, inorganic phosphorus, iron, total iron binding capacity, and hemoglobin autoxidation rate.
The obtained results can be summarized as follow:1- Hemoglobin: There was a significant decrease in hemoglobin comparing with control group while a significant increase in groups given vitamin C comparing with ISMN treated groups of the same dose.2- Methemoglobin: There was a significant increase in methemoglobin concentration was observed in all treated rat groups compared with that of the control group, while significant decrease in methemoglobin concentration was observed between groups given vitamin C comparing with ISMN treated groups of the same dose.3- Carboxyhemoglobin: Carboxyhemoglobin showed a significant increase in treated rat groups compared with that of the control group, while a significant decrease incarboxyhemoglobin in all groups given vitamin C comparing with ISMN treated groups of the same dose 4- Saturated oxygen: Saturated oxygen showed a significant decrease in treated rat groups compared with that of the control group, while a significant increase in groups given vitamin C comparing with ISMN treated groups of the same dose.5- Oxygen content:
Oxygen content showed a significant decrease in all groups compared with that of the control group, while a significant increase in the group given vitamin C+ ISMN 6.8mg/kg comparing with ISMN treated groups of the same dose.6- Hematocrit: All animal groups showed a significant decrease in hematocrit compared with that of the control group, while a significant increase in groups given vitamin C comparing with ISMN treated groups of the same dose.7- Red blood cells count: All animal groups given ISMN only showed a significant decrease in Red blood cells count while only the group given vitamin C+ ISMN 6.8mg/kg showed a significant decrease in Red blood cells count compared with that of the control group, while all groups given vitamin C+ISMN showed a significant increase in red blood cells count comparing with that given ISMN only.8-White blood cells count: All animal groups given ISMN only showed a significant decrease in white blood cells count while only the group given vitamin C+ ISMN 6.8mg/kg showed a significant decrease in white blood cells count compared with that of the control group, while all groups given vitamin C+ISMN showed a significant increase in white blood cells count comparing with that given ISMN only.9- Nitric oxide:
There was a significant increase in nitric oxide concentration was observed in all treated rat groups compared with that of the control group, while significant decrease in nitric oxide concentration was observed between groups given vitamin C comparing with ISMN treated groups of the same dose.10- Glutathione: There was a significant decrease in glutathione content was observed in all treated rat groups compared with that of the control group, while significant increase in glutathione content was observed between groups given vitamin C comparing with ISMN treated groups of the same dose.11- Catalase: There was a significant decrease in catalase activity was observed in all treated rat groups compared with that of the control group.