الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
This thesis is concerned with the analysis of six pharmaceutical binary mixtures. These mixtures contain adrenaline-procaine hydrochloride mixture, ascorbic acid-paracetamol mixture, metoclopramide hydrochloride-paracetamol mixture, amoxicillin-flucloxacillin mixture, salbutamol sulfate- guaifenesin mixture and theophylline-guaifenesin mixture. . . The thesis comprises two parts in addition to general introduction about the studied drugs and the different official and reported methods described for assay of these drugs.
Part I:
Includes spectrophotometric methods for the determination of the studied drugs, and it is comprised of two chapters.
Chapter 1:
Includes a simple, rapid, accurate and sensitive spectrophotometric method. This method is first derivative spectrophotometry, which depends on zero-crossing measurements of the first derivative spectra of the studied drugs. A validation study for the proposed procedures according to USP 2002 was performed. Beer’s law was obeyed for all of the studied drugs in the concentration range of 0.8-15, 1-10, 2-25, 1-15, 5-50, 2-17, 3-20, 4-25, 6-60, 5-50, 3-40, 6-50 g ml-1 for adrenaline, procaine hydrochloride, ascorbic acid, paracetamol, metoclopramide hydrochloride, amoxicillin, flucloxacillin, salbutamol sulfate, guaifenesin and theophylline, respectively. The detection limit range was 0.22-1.80 g ml-1, while the quantitation limit range was 0.73-6.00 g ml-1.
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The method was successfully applied for analysis of the studied drugs in pure form and in pharmaceutical preparations. Results were compared with those obtained by the official and reported methods. The t- and F-values were calculated and compared with the theoretical values, indicating high accuracy and good precision of the proposed method.
Chapter 2:
This chapter is concerned with the development of a simple, rapid and reproducible colorimetric method. This method is based on coupling reaction between the studied drugs, which are adrenaline, procaine hydrochloride, ascorbic acid, paracetamol, metoclopramide hydrochloride, amoxicillin, salbutamol sulfate and theophylline with diazotriazole carboxylic acid reagent (DTCA) in alkaline medium. The colored products were measured in the range of 378-510 nm. A study for all the reaction variables has been conducted to optimize the reaction conditions. A validation study for the proposed procedure according to USP 2002 was also carried out. Beer’s law was obeyed for all of the studied drugs in the concentration range of 1.5-20, 0.8-10, 2-20, 2-17, 3-40, 2-20, 3-15 and 3-60 g ml-1 for adrenaline, procaine hydrochloride, ascorbic acid, paracetamol, metoclopramide hydrochloride, amoxicillin, salbutamol sulfate and theophylline, respectively. The detection limit range was 0.23 – 0.91 g ml-1 while the quantitation limit range was 0.75 - 3.00 g ml-1. The molar ratio of DTCA reagent to each of the studied drugs was 1:1 and mechanisms of all compounds were suggested. The method was successfully applied for analysis of the studied drugs in pure form and pharmaceutical preparations. Results were compared with those obtained from the official and reported methods. The t- and F-values were calculated and compared with the theoretical values, indicating high accuracy and good precision of the proposed method.
Part II:
In this part the studied drugs, which are adrenaline, salbutamol sulfate and guaifenesin were analyzed by simple spectrofluorimetric methods. All variables affecting the reaction conditions were studied. A validation study for the proposed procedure according to USP 2002 was also carried out. The linearity ranges were 19-100, 37-400 and 22-150 ng ml-1 for adrenaline, salbutamol sulfate and guaifenesin, respectively. The detection limit range was 5.69 – 10.90 ng ml-1, while the quantitation limit range was 18.9 – 36.3 ng ml-1. The method was successfully applied for analysis of the studied drugs in pure form and in pharmaceutical preparations. Results were compared with those obtained from the official and reported methods. The t- and F-values were calculated and compared with the theoretical values, indicating high accuracy and good precision of the proposed methods.
The thesis lies in 215 pages and contains 65 figures, 46 tables, 8 schemes and 230 references listed as they are mentioned in the text.