الفهرس 
material and methodsOnly 14 pages are availabe for public view
 |  | from | 100 |  |  |
| | | from | 100 | | |
Abstract
The present work aimed to study the effect of chronic administration of some currently used NSAIDs, namely,
indomethacin, piroxicam and diclofenac on the rate of
mitosis and on the chromosomal pattern of bone marrow
cells of albino rat.
Bone marrow samples used through this study were
obtained from 200 albino rats. They were divided into 4
equal groups, each comprising 50 animals. In the first
group, the animals were given indomethacin at a dose of 3
mg/kg daily. In the second group, the animals were given
piroxicam at a dose of 1 mg/kg daily. In the third group,
the animals were given d{clofenac at a dose of 2 mg/kg
daily. The three groups were given the drugs for 10,20,30,
45 and 60 days. In the fourth group, the animals were give
5% gum arabic aqueous solution daily ·for 10,20,30,45 and
60 days and were used as controls. Observations of the
cell kinetics and cytogenetic changes of the bone marrow
cells were carried out on 10 rats from each group after
10,20,30,45 or 60 doses of drug administration. The method
of preparing met~ase spreads from bone marrow
according to Palmer et a1. (1972) and Giemsa
was used
stain was
prepared according to the method described by Genest and
Auger (1963).
For each rat, 500 cells were counted and the percentage
of the transformed cells were calculated. For chromosomal
analysis, 50 metaphases were examined for the
presence of any chromosomal a~omalies either structural or
numerical, and the results were tabulated and tested using
the student t test. ·The results of the present study
revealed the following :
1- The mean transformed cell percentage in control samoles
after 10,20,30,45 and 60 days were 24.28%±1.5623,23.96%
±2.0336, 23.66%±1.5762, 24.36%±1.8808 and 24.06%±1.8055
respectively and there was no significant statistical
difference between them (P·<0.05).
2- The mean transformed cell percentage in indomethacin
samples after 10,20,30,45 and 60 days were 23.96% ±
1.7489, 22.98% ± 2.147, 22.2% ± 1.9067, 20.78% ± 1.6357
and 17.96% ± 2.2125 respectively and there was a high
statistically significant reduction in t~e samples
examined 45 and 60 .days after drug administration (P
<0.001). On the other hand, the samples examined 10;20
and 30 days after the drug administration revealed no
significant statistical· difference ·in comparison with
the control (P <0.05)•
3- The mean transformed cell percentage in piroxicam
samples after 10,20,30,45 ~nd 60 days were 23.8%±1.917,
22.96%±2.1602, 21.94%±2.1338, 21.22%±1.6l79 and 18.26%
±2.2671 respectively and there was a high statistically
significant reduction in the samples examined 45 and 60
days after drug administra~ion (P <0.001). On the other
hand, the samples examined 10,20 and 30 day after the
drug administration revealed no significant statistical
difference in comparison with the control (P <0.05).
4- The mean transformed cell percentage in diclofenac
samples after 10,20,30,45 and 60 days were 24.44%--±
1.7074, 23.28%±2.0221, 22.66%±2.0773, 21.68% ± 1.9078
and 18.64% ± 2.3626 respectively and there was a high
statistically significant reduction in the samples
.examined 45 and· 60 days.after the drug administration
(P <0.001). On the other hand, the samples examined
10,20 and 30 days after the drug administration
revealed no significant statistical difference in
comparison with the control (P <0.05).
5- The total numbers of chromosomal aberrations in 500
examined cells for the controls including structural
anomalies, aneuploidy and polyploidy were as follows :
a- After 10 days
b- After 20 days
c- After 30 days .. 2 4 8
d- After 45 days 2 3 . 8
e- After 60 days. : 2 : 3 : 9
6- The total number of chromosomal aberrations for the
indomethacin samples including structural anomalies,·
aneuploidy and polyploidy were as follows :
a- After 10 days
b- After 20 days
2 : 4 7
3 3 : 9
-84-
c- After 30 days: 4 : 5 :.11
d- After 45 days 7: 9 : 15
e- After 60 days 9 13 : 19
7- The total number of chromosomal aberrations for the
piroxicam samples including structural anomalies, aneuploidy
and polyPloidy were as follows :
a- After 10 days : 2 : j : 7
b- After 20 days : 3 : 3 8
c- After 30 days : 4 5: 10
d-.After 45 days.: 8 : 9 .:14
e- After 60 days 10: 11 : 18
8- The total number of chromosomal aberrations for the
diclofenac samples including structural anomalies,
aneuploidy and polyploidy were as follows :
a- After 10 days : 2 3 8
b- After 20 days : 3 4: 7
c- After 30 days : 3 : 5 : 9
d- After 45 days : 5 : 7 : ’13
e- After 60 days 9·::U: 17
9- The chromosomal aberrations in the studied groups
examined 10,20 and 30 days revealed no significant
statistical difference ~n comparison ,with the control
(P <0.05). On the other hand, the three drugs caused
significant increase in chromosomal anomalies, structural
and numerical, after drug administration for 45
and 60 days as compared to normal untreated controls (P
<0.05).
from the previous results it may be concluded that
the slgn!iicant decrease of transformed cells occurred 45
and 60 days after drug administration for indomethacin,
piroxicam and diclofenac and the chromosomal aberrations
found were statistically significant after these therapeutic
daily administrations for 45 and 60 days. So, one may
assume that the tested drugs exhibit their effect after 45
and 60 days of administration.
In addition, the choice of any of the drugs will be
according to the sensitivity of the disease in question
and/or according to the accomodation of the patient to ~he
side effects since all drugs had nearly the same effect on
the cell··kinetics and the dhromosomal· pattern.