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Abstract
The present study was undertaken to investigate the following topics:
1- Isolation and purification of pathogenic bacteria from different sources.
2- Studying the susceptibility and resistance of bacteria isolates isolated from the patients to different β-lactam antibiotics.
3-Determination of MIC of some β-lactam antibiotics against resistant isolates.
4- Identification of highly resistant isolates.
5-Determination of the β-lactamase activity of the selected highly resistant isolates.
6- Effect of some antibiotics on the MIC of selected β-lactam antibiotic (Amoxycillin) for the resistant isolates and their β-lactamase activity.
7-Studying some factors affecting the β-lactamase production by the selected isolates.
8- Effect of some metal ions and some inhibitors on the activities of β-lactamase of selected isolates.
9-Effect of different crude oils of some plants on the growth of highly resistant isolates as well as effect of combination between thyme oil and β-lactam antibiotics (amoycillin and ampicillin) each alone on the growth of highly resistant isolates were investigated.
10- Effect of Gamma radiation on the growth and β-lactamase activity of selected oislates was obtained.