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Abstract
Summary
Freshly intact genital tracts (Total : 185) taken from slaughtered, healthy buffalo cows. Among them, 121 normal genitalia, 15 affected with inactive ovaries, 10 affected with different pathological abnormalities (excluded) 21 normal genitalia with serum from the same animals and 18 pair ovaries at various reproductive conditions. Thorough morphological examination was performed to classify the genital tract into: Estrum (Day 0-1), Met estrum (Day 2-5), Early Diestrum (Day 6-9), Mid Diestrum (Day 10-13), Late Diestrum (Day 14- 17) and Pro estrum (Day 18 - 21st ).
Pair ovaries from each genital tract excised and freed from the surrounding tissues. The length, breadth, thickness and weight from each ovary taken as usual. Trial made to calculate theoretically the volume of the ovary by multiplying its Length X Breadth X Thickness X 0.52 applying the formula of the ellipsoid.
The diameter of any corpus Luteum (CL) measured with the caliper. Cross-section of CL done to evaluate color, surface moisture and size in relation to the cut surface area of the ovary. Using a magnifying eye lens the appearance of the vascular network on the CL surface taken into consideration beside the other measurements to verify the different phases of estrous cycle. The CL enucleated from the ovary to evaluate its variable degrees of resistance and in many occasions weighed separately.
The number of surface follicles (F) and their diameters measured. The follicles classified according to their diameters into: small (< 5 mm), medium (5-8 mm) and large (> 8 mm). The follicular fluid (FF) aspirated separately from each F with 1 ml disposable Primo syringe to the nearest 0.01 ml and kept in Eppindorf tube at -20 C˚ for biochemical analysis. The volume, color, viscosity and PH of follicular fluid evaluated. After FF aspiration, some ovaries subjected for CS to measure the depth of F, which gave a relatively large amount of FF in relation to its measured diameter.
Blood samples taken at the time of slaughter from the already mentioned 21 buffaloes and sera were separated and kept at –20 Cº till the time of biochemical analysis to search for any relation between follicular fluid contents and serum profile of the studied elements. The FF in these cases taken as pool sample from all follicles in both ovaries of each cow and kept as usual.