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Abstract
SUMMARY
A total of 330 random samples of milk and some milk products including raw milk (150), soft cheeses (Damietta and Kareish cheese), ice cream (50 samples each) and cooking butter (30) samples, were collected from street vendors, farmer’s houses, dairy farms and dairy shops in Sohag City. These samples were examined for the prevalence of Helicobacter species using two selective media: Helicobacter pylori special peptone agar (HPSPA) and Columbia blood agar base.
The results revealed that 29 (19.3%), 10 (20%), 15 (30%) of the examined raw milk, Kareish cheese and ice-cream samples were contaminated with Helicobacter spp. on HPSPA. However, the incidence of Helicobacter spp. on Columbia agar was 22 (14.7%), 3 (6%), 13 (26%) in the same samples, respectively.
H. pylori was isolated in a percentage of 0.7% and 2% from the examined raw milk and ice-cream samples, respectively on HPSPA and in a percentage of 1.3% from the examined raw milk samples on Columbia agar.
In addition, other Helicobacter spp. were isolated from the examined samples as H. cinaedi which recovered in percentages of 6.7, 6 and 8% from the examined raw milk, kareish cheese and ice-cream samples, respectively on HPSPA, while on Columbia agar its incidence was 2, 6 and 6% from the same examined samples. While, H. pullorum was also isolated in a percentage of 8, 6 and 12% on HPSPA from the examined raw milk, karesih cheese and ice-cream samples, while it was