الفهرس Only 14 pages are availabe for public view
 |  | from | 168 |  |  |
| | | from | 168 | | |
Abstract
The development of arobust Agrobacterium-mediated transformation protocol requires the identification and optimization of the factors affecting DNA delivery and plant regeneration. Three different explants from three different genera (tomato, cucumber and tobacco) were used to study the effect of genotype, explant type and plant growth regulators treatments on callus induction and plant regeneration to determine for genetic transformation using antibacterium tumefaciens strain LBA 4404 and evaluate the effect of infection time and the bacterial cells concentration on transformation frequency.