الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
This investigation was carried out at the Tissue culture laboratory,
Department of Horticulture ,Faculty of Agriculture , Moshtohor during ..
1992-1995 . The main objectives of this study is to find out the possibility, of
establishing some of new grape and strawberry cultivars and Balady
strawberry plants through in vitro propagation. Also, identification of the best
ways, for enhancing production of the largest numbers for covering unlimited
requirements from these plants. In addition suitab1e methods for rooting and
acclimatization of these plants were involved . Meanwhile, evaluation of some
new cultivars of either grape or strawberry and studying the response of these
cultivars to different physiological factors as the consequences of light and
dark conditions as well as the effect of different growth regulators on growth,
development, and rooting of these plants were included. Also, the effect of
growth regulators balances on development of tissue cultured plants and at
acclimatization stage were studied.
The results can be summerized as follow :
1. Solidified Nitsch and Nitsch medium induced the best explant development
and shootlets criteria in case of grape cultivars. However, Murashige and
Skoog was preferred in strawberry plants.
2. Anderson medium proved to be unsuitable for both grape and strawberry
plants as it increased nicroses and reduced shootlets and chlorophyll.
3. Different cultivars of grape and strawberry showed a slight differences with
regard to explant development and shootlets other as different medium types
were used,
4. Shoot tips of grape and strawberry plants surpassed one node cuttings in
explant development and shootlets characters.
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5. Solid medium was supenor in growth during establishment stage and
chlorophyll while semi-solid medium state was recommended during
proliferation stage as it increased proliferation and nicroses. However, liquid
medium greatly reduced nicroses and increased growth, chlorophyll and
rooting.
6, Different cytokinin types had different effect on growth parameters of either
grape or strawberry plants. Since, kinetin and zeatin at 2 mgll level
enhanced greatly growth and chlorophyll. However, 6-benzylaminopurine at
the same level increased proliferation. On the other hand, 2 mgll thidiazuron
encouraged the highest nicroses which resulted in inducing adverse effect on
all growth parameters.
7. The lower concentration of 6-benzylaminopurine (BAP) (1.0 mg/l) and ( 2.0
mg/1 ) for strawberry and grape respectively increased growth and improved
chlorophyll however higher concentration of BAP (4 mgll) encouraged
proliferation with great level and increased nicroses.
8. Thidiazuron (TDZ) at 4 mgll for grape and 2 mg/l for strawberry plants
killed the cultured plantlets while lower concentration 0.5 and 1.0 mgll -,
enhanced good growth, proliferation, and chlorophyll .
9. Indole-3-acetic acid and naphthalene acetic acid were superior in increasing
growth and chlorophyll when compared with indole-3-butyric acid at the
same level while indole-3-butyric acid and NAA surpassed IAA in
increasing callus production, nicroses, and rooting of either grape or
strawberry plants.
10.The lower concentration of auxin (1.0 mgll) reduced callus production and
nicroses while increased both growth and chlorophyll. However, higher ones
(2.0 or 4.0 mgll) encouraged rooting.
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i 1.Continuous light and intermittent light increased both callus production and ’.
nicroses while they had harmful effect on the other growth parameters.
However, long day photoperiod ’treatment enhanced growth and chlorophyll.
On the other hand, rooting was improved greatly by the short day
photoperiod treatment.
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12.Darkening treatments (surface coverage, outer coverage, and their
combination) increased growth, chlorophyll and rooting of grape and
strawberry plants.
13.Addition of activated charcoal to the cultured medium exerted an adverse
effect on all growth and rooting parameters of the studied plants.
14.Acclimatization treatments for grape and strawberry plants were varried
greatly in the resultant rates of survival. The combination of foam, sand,
peat-moss, and loam in one treatment produced the highest survivals
followed with the combination of sand, peat-moss and loam.
It is concluded from the above that the best medium for establishment,
proliferation, and rooting of different grape cultivars was Nitsch and Nitsch ..
while Murashige and Skoog medium was suitable for strawberry plants. Also,
shoot tip explant was preferrable in both grape and strawberry plants. In the
mean time , solid medium was recommended in establishment stage while
semi-solid mediwn was suitable for proliferation stage and liquid medium
induced the best rooting in both grape and strawberry plants. Furthermore,
kinetin and zeatin encouraged the best growth and chlorophyll. However,
thidiazuron and 6-benzylaminopurine enhanced the highest proliferation in both
grape and strawberry plants . Meanwhile, lower concentrations of cytokinin
induced . better growth and chlorophyll than higher concentrations which
encouraged proliferation. In contrast, higher concentr-ations of cytokinin-like
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. (thidiazuron) up to 4 mgll in case of grape and 2mgIL in strawberry plants
stimulated domination of nicroses which affect harmfully all growth
parameters. The best thidiazuron concentrations for inducing good growth
parameters and good proliferation were 0.5-1.0 mg/l, In addition, rooting was
increased greatly by using either 2 or 4 mgIL IBA . Also, short day
photoperiod light regime increased rooting of both grape and strawberry plants.
In the same time- , combination of surface and outer coverage treatment
enhanced rooting. Meanwhile, combination of foam, sand, peat-moss and
loamy produced anexcellant survival rates.