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Abstract For this study three The main objective in this investigation was tc study the physioloeical and biochemical effects of Vitavax Captan as fu~icide~ Fluometuron and Trifluralin as herbicides, ane Drospan and Lanit as insecticidES, on Giza 69 cottar variety. lines of experiments, in the laboratory, the greenhcuse and the field WEre conducted. 1. Laboratory eXferiments : Seeds of cottcn were soaked in fungicide and hErbicides solutions in water at the rate of 1/10 of tte field recommended rate. These soaked seeds were planted, half in Petrj dishes and the other half in 10 em diameter pots. The germination percentage, radiele + hypocotyle, reot and stem lengths, aeedLf.ng lengths, and dry weigbts were all meausred. 2. Greenhouse ex!eriment: The seeds werE planted in }O em diameter clay pats and treated with the pesticides at the rate of 2/3 the field recommended rateo The pesticides were afplied individually ar in combinations. The data of germination percentagE, root length, stem length (plant hieght), dry weight/plant, and the chemical analysis of t.le cotton leaves at the age of 45 days were all recor- Ie d , Field ex~rim~nts : Field exp.was carried out in the experimental esa t Lon farm 0 f the A?;ric. Research Centre 0 f Giza dllring 1978 and 1979 3eaSOns. The pesticides were a)plied in different ~ombinations as the recommended rates and time. The effect of combined pesticides on growth and yield 0 f cotton plant were studied. Leaf a.id seed analysis for all chepri ca.l contents were dete- .rrni.ned , The results ob tad ned can be summerized in the following: - (1) The laboratory experiment~: The germiaation percentages and the radi-C:.8 + .bypocotyle Lengjhs in petry dishes were reduced b~’ soaking seeds in p€~sticide solutions, except Vitavax Ce.pt an which gave an :.ncrease. The root and ~,temlengths and dry weight/plant, of plants grown in poi s, were increased by soaking seeds ill Vitavax and }!~luomehl..ron and their combinations,while Tl’ifluralin and its ccmbination with Vitavax caused a rE duction.(2) Green house experiment: Germination percentages were significantly reduced by the application of combine d fungicide with ner» bi cides.Plant hiehgt was significantly increased only by the application of :Eluometuron alone Dry weight/plart was increased by fungicide and herbicide applications. The insecticides caused a reduction. The herbicides and Lanit treatments increased the total chlorophyll in the cotton leaves, while the furgicide and Drospan ehowed a reduction All pesticide treatments increased the total carotenoids, as comparEd to control. The pesticide applications had no clear effect on the mineral content of the cotton leaves. The total carbchydrates content was increased by the pesticide applications, except Trifluralin which cacse d a reduction. Generally, the pesticide treatments tended to The fungicide and herbicides tended to increase the reducing sugars content, while the insecticides caused a reduction. reduce the phenol com~ounds in the cotton plant compared to control. The total aminJ nitrogen was generally, reduced by the pesticide treatnents as compared to control. (3) Field eXEeriments: Germination percentages were decreased signifi~ antly by the applic~tion of Trifluralin and Fluometur) n in 1978 season, and Vitavax Captan + Trifluralin in 1979 season as compared to the contro1. The plant hight, generally, was increased by the pesticides treatments as compared with the control es~ecially at the plant age of 90 days. The increame~ t was significant o~ly at plant age of 45 days in 1979 season. The application of pesticides showed an increaas in dry weight/pl.an~ at the plant age of 45 days in 19’r9 season, as compar-ed to control, the differences weJ’e significant. At the plant age of 90 days the ef:~ect bad the opposi tl!trend with insignificant di:~ferences• The pesticides treatments had no clear effect on stand count as compar-ed to control. The differen~ es between treatments and control were not signifi~ ant. The application of herbicides and insecticides tended to increase thE yield of seed cotton in kentar/ feddan as compared with the control, while vitavax capten tended to decreasE the yield but the differences WEre not significant. The pesticidef treatments had no deffinit effect on the average of boll weight in both seasons au compared with corrt roL, The differences between the treatments were not significant. The pesticide,3 applications, generally, caused a reduction in lint p~rcentage values as compared with t .ie control. The re Iuctd.o n was more pronounced in 1’379 season. The pesticides treatments had no diffenit effect on seed index a~[ compared with control, the diffeJ’ences were not sign.:i.ficant. The applieatioJLS with pesticides, generally, showed a reduction in the earliness percentage, as conpared with the corrt ro L, the differences were significant only in 1979 lleason. Fineness (Micremire values) and strength (PJ~essely index) were lIot affected by insecticides applications The chlorophyl:. a content of the cotton leaven tended to increase by fungicide and herbicides applications at the ages of 45 and 90 days in 1978 aeuaon, while their ef:’ect was the reverse in 1979 seuson, The insecticicles caused a reduction in chlorophyll a content in 1~178season but the effect was thl~ opposite in 1979 spason. Vitavax Captan and Trifluralin reduced chloroprll b content in the cotton leaves at the ages of 45 and 90 days in both seasons as compared to the control. The insecticides increased chlorophyll b content at the age of :-10 days while at 125 days old thu results were the opposite. Fungicide and terbicides applications individue, lly or in combinaticns, increased the total chloro~ hyll content of the cotton leaves at the ages of 45 and 90 days in 1978 season, while their effect tendec. to be the opposite in 1979 season. The insecti’” cicies t generally, tende d to increase the total chlorophyll in both seasons. Vitavax Captan, Trifluralin and Flurometuron t er.ded , in general, to increase the total carotenoids content of the cotton leaves at the ea.rly stages in 191’8 season, while the effect was the reverse in 1979 aes.so n, The insecti cj des, generally, caused a reducti( ln in total carotenoids in 1978 season,while showed an increase in 1979 aeaaon; The fungicide and herbicide treatments tended to increase the total llitrogen content in the cotton leHves at the early sthges in both seasons as compared wi trh the contro 1. ThE insecticides however, had no cLua.r trend. Vitavax capten Trifluralin and Fluometuron caused an increase of -;he total P content of the CO”itonleaves at the e~,rly stages in 1979 season, as - compared with the contr)l. Drospan treatments showed a reduction of P content at the ages of 110 and 125 days in both seasons. Generally, pota3sium content of cotton leaves was increased at th? age of 45 days in both seasons, by using the fungicide and herbicides. The insecticides however, h~d no clear effect on potassium content. The fungicide and herbicides tended to increase the total carbohyirates in the cotton leaves at the age of 45 days in 1978 season, while at 90 days the carbohydrates content was decreased, as compared to control. The insecticides tended to decrease carbohydrates in 1978 season. All pesticides however, had no clear effect in 1979 season.The total phenols content in the cotton leaveswas increased at the ag~ of 45 days in 1978 by applying fungicide and herbiiides, while at the age of 90 days the same mentioned pesticides caused a reduction. In 1979 season, the resllts were the opposite. The insecticides showed in ge~eral, a reduction of total phenoLs as compared with t 1.e control. Genera.lly, the polyphenals content of the cet uon leaves, at the e~rly stages, was increasee by using the fungicide Me, herbicides in both seasons as compared with the cOlltrol, while the insecticides showed the opposite effE~ct. Vitavax captan, Trifluralin and Fluometuron, genl~rally, increased thE! reducing sugars in the cotton leaves at the ea:~ly stages in both seasons as ’~omparedvv:i th the contra 1. Moreover, the insecti c:Ldes caused a reduct:.on in reducing sugars in both sea:Jons The fungicide ahd herbicides and the Lns ect Le cf.d as applications t ended to decrease the total amino nitrogen content of cotlicn leaves at the age of 45 day 3 as compared with the control in both seasons. Seed oil conterr~ and quality was not affected by the pesticide app.l.i.catd ona. |