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Abstract The causal pathogen of damping-off fungal diseases of Dodonaea viscosa and Thuja orientalis as well as those of root rot of Acalypha wilkesiana and Hibiscus rosa-sinensis were identified in nurseries of three governorates tested. Pathogenic capability of the pathogens and losses in stand and plant growth as well as several measures of control were determined. The main findings of this study may be swnmarized as follows: (1) Alternaria sp., Fusarium semitectum, F. solani, Fusarium sp. and Rhizoctonia solani were isolated at different frequencies from roots of Dodonaea and Thuja seedlings of the three governorates nurseries tested. Also, Botryodiplodia sp.and Helminthosporium sp. from Dodonaea as well as Macrophomina phaseolina from Thuja were isolated. R. solani and F. solani showed the highest frequency in case of Dodonaea and Thuja, while that of F. smitectum was the least. (2) Isolation trials from Acalypha and Hibiscus root rots yielded Alternaria sp., F. solani, Nigrospora sphaerica and R. solani at different frequencies. While, Botryodiplodia sp. and Fusarium sp. were only isolated from Acalypha as well as Aspergillus sp., Curvularia sp., F. semitectum, Mucar sp. and Pythium splendens from Hibiscus. F. solani gave the highest mean frequency in case of Acalypha (64.20%) and Hibiscus (34.60%), followed by R. solani with the two shrubs tested. Also, F. semitectum and P. splendens recorded the moderate degrees of frequencies in case of Hibiscus. (3) F. semitectum, F. solani, Fusarium sp. and R. solani were found to be pathogenic fungi to Dodonaea and Thuja since they significantly increased pre-and post-emergence damping-off than the control (without fungus). R. solani and F. solani caused the highest percentages of pre- and post-emergence damping-off as well as the lowest survivals ones with the two shrubs tested. (4) F. semitectum, F. solani and R. solani in case of Acalypha and Hibiscus as well as Botryodiplodia sp. on Acalypha and M. phaseolina and P. splendens on Hibiscus were pathogenic as they significantly increased infection (%) than the control (without fungus) in pathogenicity tests. While, Alternaria sp. and N. sphaerica in case of Acalypha and Alternaria sp., Aspergillus sp. Curvularia sp., Mucar sp., and N. sphaericea showed no effect on shrubs seedling. (5) Soaking seeds of Dodonaea and Thuja for 15 min in Topsin M or Rizolex- T was effective measure in decreasing pre- and post-emergence damping-off and increasing survival seedlings, grown in soil infested with F. solani or R. solani. The fungicide Maxim was also effective with only Dodonaea and Vitavax/Thirum with Thuja. The bioproducts Plant Guard and Rhizo-N, generally, gave variable effective results in this respect. Plant Guard however, was better than Rhizo-N in case of F. solani and the opposite result was true with R. solani. On the other hand, Thymus vulgaris and Cymbopogon citratus were significantly the most effective plant extracts tested in decreasing pre- and post-emergence damping-off as well as improving seedling survivals. (6) Peat moss or peat moss+sand as planting media for Dodonaea and Thuja in the presence of the soilborne fungi F. solani or R. solani were generally effective mean in decreasing damping-off diseases. (7) Evaluation the efficacy of the fungicides (Rizolex- T, Tachigaren, Tecto, Topsin-M and Vitavaxffhiram), the bioproducts (Plant Guard and Rhizo-N), the plant extracts (Cymbopogon proximus and Majorana hortensis) and the plant hormones (lAA, IBA and NAA) as dip treatment for Acalypha and Hibiscus cuttings, when planting in soil infested with F. solani, on the disease incidence and seedling growth parameters was as follows: A-. All treatments tested decreased percentages of the disease incidence than the control in all soil types. Topsin-M, however, gave significant decreases than the control in case of Acalypha and Hibiscus, followed by Rizolex- T with only Hibiscus. Plant Guard, Majorana hortensis extract and IBA (Acalypha) or NAA (Hibiscus) were the better bioproducts, plant extracts and plant hormones, respectively. B-. All treatments tested, except Cymbopogon proximus and IAA in some cases with Hibiscus, significantly increased leaves number per seedling of Acalypha and Hibiscus. Topsin-M, Majorana hortensis and (NAA and IAA) were the best treatments among the tested fungicides, plant extracts and plant honnones, respectively. On the other hand, peat moss was the best soil type in increasing leaves number/seedling. C-. The height of Acalypha and Hibiscus seedlings was significantly increased using all treatments tested except Rhizo-N and Cymbopogon proximus extract in sand and clay+sand soil as well as Majorana hortensis extract in sand soil. Rizolex- T, Topsin-M and VitavaxlThiram were generally the best fungicides tested in increasing seedling height. Plant Guard, Majorana hortensis extract, NAA in case of Acalypha or IBA with Hibiscus and clay+sand soil were superior in improving height of seedlings than the other bioproducts, plant extract, plant honnones and soil types tested, respectively. D-. Topsin-M (followed by Rizolex- T in case of Hibiscus) produced the highest number of branches Acalypha and Hibiscus seedling and the increases than the control was always significant Plant Guard, Majorana hortensis and rnA were superior than Rhizo-N, Cymbopogon proximus and the other plant honnones• respectively, in increasing number of branches per seedling. Peat moss+sand in case of Acalypha as well as clay-sand followed by peat moss with Hibiscus were the best soil types in increasing this plant parameters. E-. Roots length of Acalypha and Hibiscus seedlings was significantly increased using all treatments tested in case of sand, peat moss and peat moss+sand soil types. Topsin-M, Plant Guard, Majorana hortensis and IBA (Acalypha) or lAA (Hibiscus) were superior treatments in increasing root length among the fungicides, bioproducts, plant extracts and plant hormones tested, respectively. On the other hand, peat moss+sand (Acalypha) and peat moss (Hibiscus) were the best soil types in improving root length. F_. Root weight/seedling was increased by each one of the treatments tested than the control (without treatment). Topsin-M and Vitavax/Thiram (Acalypha) as well as Topsin-M followed by Rizolex-T in case of Hibiscus were generally the best fungicides in improving root weight/seedling. Also, Plant Guard, Majorana hortensis extract and IAA in case of Acalypha as well as NAA with Hibiscus were the best treatments among the bioproducts, plant extracts and plant hormones tested. On the other hand, clay+sand and sandy soil were superior soil types in increasing root weight/seedling in case of Acalypha and Hibiscus, respectively. G-. All treatments tested significantly improved seedling weight than the control in case of both Acalypha and Hibiscus shrubs. Topsin-M followed by Rizolex-T, Plant Guard, Majorana hortensis extract, IBA and clay+sand were found to be the best fungicides, bioproducts, plant extracts, plant hormones and soil types in increasing seedling weight. In contrast, the fungicide Tecto, the hormone IAA and sandy soil were the least effective treatments in this respect. (8) Effects of dip Acalypha cuttings in each one of 5 fungicides, 2 bioproducts, 2 plant extracts and 3 plant hormones on disease severity as well as root number and length of seedlings growen for 10, 17 and 24 after planting in natural or sterilized soil uninfested or infested with F. solani are as follows: A. Sterilized soil (sand+clay), uninfested or infested with F. sol ani: Topsin-M completely prevented the fungal infection and greately improved root number and length/seedling in all periods tested, followed by Vitavax/Thirarn and Rizolex-T. Rhizo-N, Majorana hortensis extract, all hormones tested were found to be of great importance in decreasing disease severity and in improving roots number and length/seedling. rnA, however, was the best hormone in this respect. B. Natural soil (sand+clay), uninfested or infested with F. solani: Topsin-M significantly increased roots number and length/seedling and completely prevented fungal infection during the three periods tested, followed by Vitavax!fhirarn in most case. Rhizo-N, Majorana hortensis extract and IBA were, however, superior than the other bioproduct, plant extract and plant hormones tested in decreasing disease severity and in improving root growth parameters. In this respect, all plant hormones tested increased significantly root length and numbers per seedling as well as decreased disease severity than the control. (9) The efficiency of some fungicides (Rizolex-T, Tachigaren, Tecto, Topsin M and Vitavax/Thiram), bioproducts (Plant Guard and Rhizo-N), plant extracts (Cymbopogon proximus and Majorana hortensisy and plant hormones (IAA, IBA and NAA) as dip treatment for Acalypha cuttings on total sugar and phenol contents in seedling roots, grown in sterilized and unsterilized (normal) sand+clay soil infested with F. solani was as follows: A. Total sugar contents in roots of Acalypha seedlings grown in sterilized soil (without infestation) were equal in value with all treatments tested and the control. While, they always decreased than the control in roots of seedlings grown in sterilized or unsterilized soil infested with F. solani and in unsterilized soil without artificially infestation. Among the fungicides and the other treatments tested, Topsin-M, VitavaxfThiram and Rizolex- T gave the lowest amount of the total sugar contents in seedling roots, while the opposite result was found with the fungicides Tachigaren and Tecto. Plant Guard decreased sugar contents more than Rhizo-N of sterilized soil, while the reverse was occurred in case of unsterilized, uninfested or infested with F. solani. Also, Majorana hortensis extract and NAA were effective treatments than Cymbopogon proximus and the other hormones, respectively, in most cases. B. Total phenol contents in roots of Acalypha seedlings grown in sterilized soil (without infestation) were equal in value with all treatments tested and the control. While, they always increased than the control in roots of seedlings grown in sterilized or unsterilized soil infested with F. solani. The highest phenol contents were found in case of Topsin M, Rizolex-T and Vitavaxffhiram, followed by the bioproducts (Plant Guard and Rhizo-N), Majorana hortensis extract and IBA plant hormone in sterilized, infested soil and unsterilized, uninfested or soil infested with F. solani. |