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Abstract
Mastitis is the most prevalent and expensive disease on a dairy farm.
In the present study different bacteria were isolated from 111 QMS out of 126 collected from cases of clinical mastitis in Beni-Suef governorate.
While the prevalence of mycoplasmal mastitis was studied in a large dairy herd in Germany using culture methods confirmed by species specific PCR. The specificity of the used species specific PCR has been proofed by alignment of each primer pair using a multiple sequence alignment computer program with the sequences of reference strains of Mycoplasma species most commonly isolated from milk retrieved from the GenBank. In addition to sequencing of PCR products of one field strain from each Mycoplasma species detected in the current study.
A newly developed rt PCR has been used in comparison to culture method for 36 QMS which was able to detect bacterial loads as low as 100 colony forming units/ml of milk. The ability of this system to detect M. bovis in group animal (16-17) milk samples and tank milk has been proofed. The diversity of some M. bovis isolates and all M. californicum isolates has been detected using RAPD fingerprinting technique.