الفهرس 
Active constituentsOnly 14 pages are availabe for public view
 |  | from | 293 |  |  |
| | | from | 293 | | |
Abstract
Medicinal plants containing narcotic principles en, and are still, the subject of researches of vestigators. This was attributed to their mediiuse as analgesics, hypnotics, stimulants, anti- br die and in treatment. of cough and diarrhoea. narcotics are produced by several plants belong- br the family :Papaveraceae. J’a.l?a’ye:xrum L. br e most important plant -#119;- morphine, codeine, tine, thebaine, Inpaverine and narceine are the important alkaloids present. br In the present wor;k, a phytochemical study of culti vated in Egypt using two, ea of seeds (200 and 4) obtained f~om Au~tralia This study includes 2 parts :- br Preliminary phytochemical screening of the different organs of J;.a..Eave.r .. s.omnife~ L. (Sample 200 and 4) : br Established the presence of carbohydra.tes and/or cos1des, free and combined flavonoids, catechol tannins, br ~/or triterpenes and alkaloids and/or nitroobases in all the examined organs except the seeds tree -#102;-#114;-#111;-#109; tannins and flavonoids. br ProJdma t e Atl .. §,Jxs ip : br selective organic solvents wd ~ investigation of the successive extracts. br The· different organs were extracted with successive of increasing polarity viz.: petroleum (b.p. 60 - 8000), ether, chloform and alcohol 95 %. extracts of the different organs were sere ened for rconstituents and it was revealed that steroids and/or br erpenoid compounds were present in the petroleum ether, r extracts of the different organs and alcohol extracts ~ fruits and s~eds; while carbohydrates and/or glyeo- br s were present in ether, chloroform and alcohol extracts he different organs. Flavonoid compounds were found in alcohol extraotsof all the organa except the seeds; and/or nitrogenous bases are present in all the petrol~ ether and ether extracts of the catechol tannins were found in the alcohol extracts br the different organs except the seeds. br The percentage yield of extractives were determined the different DrSPns. br rIll - ~ve s:t iAfl.tj..Q~ oJ~he l,il’id f):’.§-tio}! : physical and chem-ical cpa,raeters of both samples (200 and 4). br steroidal and/or tri terpenoidal loonstituent of the br s of both samples revealed 5 spots which were separated olumn chromatography. br The first compound A was isolated as white trans- br t waxy substance which was not identified, substances de; the physical and chemical studies on them reveal ed t, they are saturated aliphatic alcohols, one of which identical to 10 Nonac .s nol. Substanoe D is identiiB-sitosterol, while substance E melted ~t 125°0 and br e positive LiberInaJll1’g test. br I s,tUgy .of tpe. f’~£id£...gL_~~ see-lt;\B of_ both saml?les .l~..oQ. ap.d 4) : br GLe investigation of the methyl esters of the fatty aids using .polyethylene glycol (10 %) showed that the two pIes contained the same number of fat ty a.cids viz. inoleic (62.66 %)., palmitic (19.49 %). oleic (15.47 %), \ br (2,1.37 %), palmitoleic (0.17 %). myristo:Leic %) and behenic (0.15 %) and their percentages early the same in both samples. br Ipvesti£?tion or am~o ~cids and proteins. present in the seeds of Kapaver ,sopm;i.:t?rum L. br were identified by subjecting the to PC, TLC and two dimensional TLC which revealed the presence of lysine, arginine, br acid, glycine, glutamic acid, threonine, d:-amino butyric acid, methionine, vaJ..ine, phenyl alanine, leucine and/or isoleucine. Cysteine was present onJ.y in sample 4. br Proteins were prepared -#102;-#114;-#111;-#109; the seeds of both samples (200 and 4) and subjected to acid hydro- br lysis, as well as, alkaline hydrolysis. The hydrolyysates were examined as in the case of the free amino br acids. The results revealed the presence of cysteine, lysine, argenine, aspartic acid, glycine, serine, glutamic acid, threonine, alanine, proline, ~butyric acid, tyrosine, methionine, valine, leucine and/or isoleucine, phenyl alanine and .3 unidentified amino acids. Tryptophane was detected in the alkaline hydrolysate. br and inve§ti”Kation of. s.om.e f).av •. on~ compormd s of’ the f1 ower-petal s of Papavel.: br sonmif’ erum L. : br Investigation of’ the flavonoid contents of the br by applying PC and TLC using different sollrevealed three spots of’ flavonoid components and C were separated by PPC -#102;-#114;-#111;-#109; the lacetate extract. Further study on the three f’lavo- br a1 compounds showed that one of’ them was a free flavooand tha other two flavonoids were glycosides. The vonoidal glycosides were sUbjected to hydrolysis. the moieties were separated as needle crystals. The of glycosides Band C had the same m.p_ (222-223 ehroma.tQ~ln.io p8.ltteJm. of aglycone A using TLC. br The chromatographic study of the sugar moieties by TLC revealed the presence of glucose in case of br B and C. The structure of the isolated flavoooid compounds were detennined on the basis of preliminar ~emical test, specific colour tests, U.V. absorption. I my be concluded that the aglycone A is possibly 3., 4·, 7-trihydroxy-3,5-dimethoxy flavone or 3’,4’,7 -trihydroxy .. -methoxy flavone. Glycoside B is probably a secondary br de of glycoside C. Consequently, glycosides B and C t probably ’7 ,3 t -dihydroxy-5-methoxy flavone 4 ’-0_ es or 7 ,J!..dihydroxy-J, 5-d:iJnethoxy flavone 4 t -0- br Study of the alkaloidal content of Papav,.?r.. br -~----~~~~_. _._- .•.•... br §.9mnif’erwn L. growing in Egypt at the br nvestiga tion of the alkaloidal content of Pa1?ave,£ somniferum L., at the different stages of growth : br ..•. . - br Using TLC and solvent system : chloroform - methanol (9 : 1). The following results were obtained. br Most of the different organs at different stages of ~wth revealed the presence of narceine, morphine, codeine,;thebaine, naroctoline and papaverine, br Before forma. tion of flower, the root contained the largest number of alkaloidal spots when compared with other organs. br At the flowering stage the highest number of alkaloidal spots were present in the flowers followed by stems, br . leaves and roots. br t the mature stage. the greatest number of alkaloidal ots were found in the latex followed by the pericarp the fruits, while· the seeds showed the lowest· number br content of the different organs of p’aj?aver br ~...--. . - .”.. br so~iferum L. grown in Egypt at the different stages of br ’. ......----_._-------- br growth (Semple 200) : br It was found that the morphine content differed titatively in the different organs at the different es of growth,. In YOIDig plants·; the roots contained br highest percentage (O~32) followed by stems (0~09) and aves (0.06) in succession. At the beginning of nowering age (bud form), the h1ghe st p$rcen tage of morph.in,e was in e flower buds (0.33) fo~lQWe9- by sterns (O.26), leaves br .19) and roots (0,02). At full flowering, the highest br of morphine was in the nower’lJ .(O.47)f.:Q:!.lowe-lt;i by br 9mB (0.35), leaves (0-.25) and roots (0 .• 22), respectively. ing fonnat.1onof’ fruit the morphine content was accumuuted in the fruits (0.59) followed by stems (0,4),’ leaves O~26) and roots (0.2). When~he fruit became. fully grown ut unripe the morphine percentage of all organs decreased br . , br bruptly’in the fruits (0-,2S), roots (0..09), stems (0.06) and lea.ves (0.03), respectively. However, the seeds showed he absence of morphine. br •• STUDY OF CERTAIN FACTORS WHICH MAY INCREASE THE GROWTH” AND YIELD OF P.A:P.A”lTER SOMND’tRtJM L.: br *” • •• ...-.-.... br - .the effe 9..t....9f NPK .. f.ertil:lze:;:.s on the -’.owth and yield of PaRay~r pomniferum L. : br The results of this investigation showed that the the two samples were obtained ma~nly by of P2K2” The yield of capsules. seeds, br x and the percentage of alkaloids were greatly affected the different fertilizers especially by the treatments taining Nwith PK, e.g. N2P1K1 and NlP~Ka .• How~ver N’1~lKl e.d S’ign.1£iean’t. !nonaae. in codeine content on the ense of morphine only in sample 200. br Sample 4 showed the same results but here P2K2 gave re significant results in the yield. br ha ter VIII - §Judy}he effec.t _ qf •. certain othe,.!’” •.. :tactor,:S on the growth and yield of Papave~ br sonmiferu.m L. : br ...• ’’’.. br T.l1e e!.f ct of micr?~~l_~mt3l1t~n_(sa.m’pl~ 20QJ :. br It was found that treating the plant of:t:apav.e.r br erum L. with 0.07 % of micro-element Zn gave an br ase in the growth and yield of the plant especially umber of branches and capsules, weights of the cappseeds and the percentage of alkaloids in the br Treating the plant of PaE6:.ver _~..9.lP.tliferuJn L. with B-indole acetic acid gave an increase in the dry weights of entire plant and its organs br and dry weights of the capsules~ as slight increase in the length of the plant, number of branche s and capsules were decreased an the control. The yield of the plant viz.; fresh and weights of capsules, dry weight of the seeds end the content of capsules were decreased than in the