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العنوان
Phytochemical and Biological Studies of Heteroxenia fuscescens Soft Coral from Rea Sea/
المؤلف
Seha, Mena Magdi Thabet.
هيئة الاعداد
باحث / مينا مجدي ثابت سيحه
الموضوع
Animal drugs. Bacteria - resistance. Antibiotics.
تاريخ النشر
2011 .
عدد الصفحات
p 268. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الصيدلة
الناشر
تاريخ الإجازة
6/1/2011
مكان الإجازة
جامعة بني سويف - كلية الصيدلة - عقاقير
الفهرس
Only 14 pages are availabe for public view

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Abstract

The search for novel compounds from marine organisms has been intensified since 1970. The collection of organisms from the oceans provided us with a largely untapped resource of a range of unique structure classes and novel compounds. The Red sea represents one of the most promising areas as a source of medicinal natural products as it considered a virgin area that full of secrets.
The soft coral Heteroxenia fuscescens collected from the red sea, Egypt was investigated for its chemical constituents and biological activity. Fresh organism (1 Kg) was extracted using 70% ethanol. The ethanol extract was partitioned between hexane, chloroform and ethyl acetate. GLC analysis of the unsaponifiable part of the lipid content of the hexane fraction revealed the presence of two sterols namely, β-Sitosterol and Sigmasterol in addition to a series of normal hydrocarbons ranging from (C10-C28). The ethyl acetate fraction was repeatedly chromatographed over silica gel and Sephadex LH-20 columns. The isolation process yielded five compounds 6-hydroxy-α-muurolene, gorgosten-5(E)-3 β-ol, 1-nonadecyloxy-2,3-propanediol, (2S,3R,4E,8E)-N-hexadecanoyl-2-amino 4,8-octadecadiene-1,3-diol and Sarcoaldosterol A. The structures of the isolated compounds were established based on NMR measurements (1H NMR, 13C NMR, 2D NMR) and mass spectrometric (EIMS) data.
The pharmacological screening of the alcoholic extract showed significant anti-inflammatory, antipyretic, analgesic and antioxidant activity. In addition the alcoholic extract has showed promising cytotoxic activity against several cancer cell lines. The alcoholic extract showed antimicrobial activity against gram-positive, gram-negative bacteria and fungi. The isolated compounds showed antimicrobial activity against tested bacterial and fungal strains.
On the other hand, the coral distribution and diversity at Hurghada (the sample collection site) have been studied in order to obtain the hard and soft coral coverage in the area. The cover percentage of the coral reef community was estimated at the locality by using the standard method (the line intercept transect).
The morphological and histological studies have been performed to follow the general external and internal structure of soft coral Heteroxenia fuscescens
Keywords: Red Sea Soft Corals, Heteroxenia fuscescens, Ecological studies, Muurolene, Anti-inflammatory, Antifungal