الفهرس 
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Abstract
Incidence of H. contortus infection among sheep was studied at Monieb abattoir in Giza Governorate during June 2003 to May 2004 and revealed that 420 out of 1344 abomasa (31.25%) contained H. contortus worms. Partial purification of crude adult H. contortus antigen through gel filtration column chromatography resulted in 3 peaked chromatograms. Fractionation of H. contortus antigens through SDS-PAGE and immunoblot techniques showed relatively large numbers of different protein bands. Fifty nine random faecal and abomasal samples of sheep at Monieb abattoir were collected and examined individually and the corresponding serum samples of them were collected and subjected to ELISA using partially purified H. contortus antigen peak II (contained 26 kDa). The results revealed that (59.3%) faecal samples were found positive to Family Trichostrongylidae eggs. Also (55.9%) abomasal samples contained H. contortus worms and (57.6%) serum samples were found positive. Three groups each of 3 lambs, aged from (3 - 5) months old, were used as control negative group; control positive group which was infected orally with 10 000 L3 and immunized group which was injected subcutaneously with 3 doses of 50 ug of partially purified H. contortus antigen peak II as a vaccine, on 0, 14 and 28 days and challenged on the 43th day post immunization orally with 10 000 L3. Reduction of the FEC (41.4%) and of the mean worm burden (84%) accompanied with slightly higher PCV and significantly higher antibody levels of the immunized group were recorded compared to those of the control positive group.