الفهرس 
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Abstract
The aim of this study is to prepare monoclonal antibodies through production of hybridomas secreting specific antibodies against rabies virus.In this work four hybrids secreting specific monoclonal antibodies against rabies virus were prepared. Immunized BALB/c mice were tested for the anti-rabies titer using ELISA. This is done to select high responder mice for the fusion experiments.Spleen cells were harvested from the well immunized mice to be used for fusion with myeloma cell line using polyethylene glycol 1500, 40% concentration as a fusogenic material. Hybridomas (spleen-myeloma cells fusion) were selected from unfavorable fused cells by using HAT selective medium. Twelve days post fusion produced hybrids were screened for production of monoclonal antibodies against rabies virus using ELISA.Four positive hybridomas producing monoclonal antibodies against rabies virus were successfully produced. The two hybridomas were cloned successfully. Four stable clones were successfully produced from the parent hybridomas. Using Western Blot technique the specificity of the produced hybrids was confirmed. All prepared hybrids produced IgM isotype.The inactivated rabies virus vaccine was emulsified with Freund’s adjuvant. Primary intraperitoneal immunization of BALB/c mice was done using emulsion containing rabies virus vaccine conjugated with complete Freund’s adjuvant. Booster doses were given using vaccine conjugated with incomplete Freund’s adjuvant. The final immunizing dose was rabies virus vaccine only.