الفهرس 
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Abstract
Experimental infection with Mycoplasma gallisepticum .The experiment was designed to investigate the role of F vax, 6/85 and oil adjuvant MG vaccine in protection of chickens against experimental infection with Mycoplasma gallisepticum S6 reference strain so 200 Hubbered chicks at one day old were divided into 4 large group and each sub divided to 2 groups ( total 8 groups) as follows:-1-First group 30 birds were vaccinated by intraoccularly at fifteen-days-old with Fvax 2- second group 30 birds were vaccinated by intraoccularly at fifteen-days-old with Fvax and challenged intranasally at 30 days old S6 MG strain. 3- third group:- 30 birds were vaccinated by intraoccularly at fifteen-days-old with 6/85 4- fourth group :- 30 birds were vaccinated by intraoccularly at fifteen-days-old with 6/85 and challenged intranasally at 30 days old S6 MG strain. 5- fifth group :- 30 birds were S/C inoculated with 0.5ml of MG oil adjuvant vaccine per bird.6-sixth group:- 20 birds were S/C inoculated with 0.5ml of MG oil adjuvant vaccine per bird. and challenged intranasally at 30 days old S6 MG strain.Control birds7- seventh group Comprising 25 birds were challenged intranasally at 15 days old with S6 MG strain.8-eighth group Comprising 25 birds Kept as a control negative. Isolation of Mycoplasma gallisepticum from lung and trachea of chicks vaccinated with live and dead vaccines and control (non vaccinated/challenged) before and post challenge. ELISA test for detection of antibodies to Mycoplasma gallisepticum in sera.Found that mean OD increase in challenged than Without challenged in case of Fvax and 6/85 vaccine mean OD decrease in challenged than Without challenged in case of Oil adj vaccine. PCR was used for the detection of M. gallisepticum in different groups of the experiment.Random amplified polymorphic DNA (RAPD) technique confirmed the presence of F strain in vaccinatedand 6/85.