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Abstract The bacteriological examination of 440 milk samples collected from mastitic and apparently normal cow’s was carried out and showed that 293 samples were bacteriologically positive with a percentage of 66.5%, 105 out of them proved to be Klebsiella spp., 96 recovered from mastitic milk and 9 isolates recovered from apparently normal milk. Klebsiella pneumoniae subsp. pneumoniae was the most prevalent isolate. Most of Klebsiella pneumoniae subsp. pneumoniae were highly virulent and highly pathogenic isolates. CPS of Klebsiella pneumoniae isolates gave about 10 – 13 bands ranges in their molecular weight from 15.5 KDa to 106.29 KDa. Immunization of mice with CPS revealed a detectable protective value of antiCPS against homologues and heterologous strains of Klebsiella pneumoniae. Antibiotic sensitivity test proved that: All isolates were highly sensitive to enrofloxacin; gentamicin and norfloxacin. Most of isolates were resistant to erythromycin, tetracycline, amoxicillin and ampicillin. |