الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 162 |  |  |
| | | from | 162 | | |
Abstract
Molecular characterization of the Notch gene in Pectinophora gosstpiella (Lepidoptera- Gelechiidae).
By
Ahmed Mostafa Isa
Doctor of philosophy, scientific channels program between Minia University in
..,.. Egypt and University of California, Riverside in USA, 1998.
The goal of this work is to look for the highly conserved Notch gene in the pink bollworm (PBW), so that a transgenic conditional dominant lethal mutation from that gene could De induced in the insect genome. Thereafter, the mutants will be released to compete with the wild insect population and collapse it.
Four main DNA fragments constituting the intracellular domain of the Drosophila Notch gene (N 12, N34, N56, and N78) were PCR-amplified and cloned. A positive southern blotting experiment was carried out to a partially digested PBW genomic DNA using the Drosophila N78 labeled probe. This indicated the presence of some kind of sequence homology between the
Drosophila N78 probe and a DNA fragment within the PBW genome. This result was later assured by another positive southern blot for the ND12 fragment that was amplified from the PBW genomic DNA using the two degenerate and highly conserved primers ND1 and ND2. The ND12 is expected to be a small fragment within the cdc10 repeats of the PBW Notch gene. For some reason, screening of the PBW genomic DNA library with a Drosophila Notch fragment was not successful. On the other hand, the high sequence similarity between the PBWWND12 and the known Notch genes was encouraging to use the Drosophila modified Notch primers N7B and N8B to amplify the PBW-N78B fragment which was then cloned and sequenced. The PBW-N78B is expected to constitute the
..,. whole cdc10 repeats domain in PBW. The matching results between the PBWWN78B and the Drosophila Notch gene were 78.9%, 92%, and 77.3% in 111 bp, 191 bp, and 112 bp fragments respectively. This indicates the certain availability of the PBW Notch gene that I named ”Potch”. Future work will complete describing the gene.