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Abstract The pretreatment of alperujo ended with extracting 4.9% residual olive oil. The crude fib er, total carbohydrate and oil content were decreased in fungal treated samples by 36, 62 and 96%, respectively. On the other hand, an increase in ash and protein contents was noticeable recording 153.2% and 47.6%, respectively, while there was no change in phenol content The enzyme activity of xylanase and CMCase reached its maximum activity at 30 QC after 5 days recording 2.12 and 2.45 IU/ml, respectively. The maximum activity of both enzymes produced at 55 ”C recording 1.13 and 3.40 IU/ml for xylanase and CMCase, respectively, showing they are thermophlilic. The maximum specific activities of xylanase and CMCase (2.53 and 3.22 ID/m I , respectively) was obtained when alperujo( AL) was only soaked in distilled water for 18hrs. The alkali pretreated alperujo followed by acid was the best pretreatment trial to produce protein (20.7mg protein per g waste) and monomer sugars as xylose (26.5Ilmol per g waste), and glucose (51.7Ilmol per g waste) when fungus grown on the pretreated waste with no additives. Alperujo methanol and ethanol extracts have highest antagonistic effect against E. carotovora subsp. carotovora at 20.25AU and 17.36AU, respectively. Second in order was R. solanacearum, which was antagonized with both extracts at 13.44AU, followed by E. arnylovora that was also affected with both extracts at 2.78AU. |