الفهرس 
Kidney
NephrotoxicityOnly 14 pages are availabe for public view
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Abstract
The present work was undertaken to investigate the beneficial effect of L-arginine and/or taurine in three different doses on gentamicin-induced nephrotoxicity in rats. Rats were divided into sixteen experimental groups. 1. Normal rats were randomly divided into eight groups and were given L-arginine and /or taurine to demonstrate their safety. 2. L-arginine and /or taurine were administrated simultaneously with gentamicin to the normal rats in the other eight groups to investigate the benefit effect of these amino acids against gentamicin nephrotoxicity for a period of consecutive 10 days. After the last injection, rats from each group were kept individually in metabolic cages for collection of 24hour urine samples. Body weight of rats, absolute and relative kidney weights and urine volume were determined. The following parameters were measured: blood urea nitrogen and serum levels of creatinine, and uric acid. Serum and urinary levels of total protein, sodium, potassium and activity of alkaline phosphatase. Renal content of malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH) in kidney tissue and whole blood, also, activity of erythrocyte Cu, Zn-SOD were determined. Histopathological examination of renal sections was also carried out.
The present study demonstrated that treatment by L-arginine and / or taurine caused a marked improvement on renal deterioration induced by gentamicin. L-arginine and / or taurine treatment reversed the decrease in body weight as well as increment in the blood urea nitrogen and serum levels of creatinine and sodium and urinary levels of potassium, alkaline phosphatase, total protein, renal content of MDA, NO, kidney weights (absolute and relative) and urine volume. Also, treatment by these amino acids counteracted the reduction in serum levels of total protein, alkaline phosphatase, potassium and urinary levels of sodium, renal and whole blood GSH and erythrocyte Cu, Zn-SOD which induced by gentamicin nephrotoxicity. Treatment by taurine especially at dose 3 (1.5 g/kg/ b.wt) and combined amino acids L-arginine (1.6 gm/ kg/ b.wt) and taurine (0.75g/kg/b.wt) had the most improvement on gentamicin nephrotoxicity.
In conclusion: L-arginine and / or taurine tested doses did not show negative physiological effects throughout the time of the study (10 days), it may point to relative safety of these amino acids for human use. Treatment with L-arginine and / or taurine resulted in improvement in the biochemical and histological changes of gentamicin nephrotoxicity