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العنوان
Interaction of root-knot nematodes and fungal diseases on potatoes /
المؤلف
Mohamed, Mohamed Zaky El-Shennawy.
هيئة الاعداد
باحث / Mohamed Zaky El -Shennawy Mohamed
مشرف / El-Said Z. Khalifa
مشرف / Mohamed M. Ammar
مشرف / El-Shawadfy M. Mousa
الموضوع
Root-knot nematodes. Potatoes.
تاريخ النشر
2012.
عدد الصفحات
120 p. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
1/1/2012
مكان الإجازة
جامعة المنوفية - كلية الزراعة - AGRICULTURAL BOTANY
الفهرس
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Abstract

Potato (Solanum tuberosum L.) is considered one of the most
important vegetable crops in Egypt and in the world, as well as its
importance in local fresh consumption and use in food processing.
Potato plants are suffering from many diseases during all
stages of growth. Fusarium wilt, black scurf and stem canker and
root-knot nematode are the most popular diseases, which cause
considerable losses in tuber yield especially in their (disease complex).
Results obtained from the present investigation could be
summarized as follows:
1. According to potato growing areas, there is a variation in
percentage of occurrence of Rhizoctonia solani and Fusarium
spp. in four governorates. The highest percentage of R. solani
was recorded in Kafer El-Zayat (68.3%), while the highest
percentage of Fusarium spp. (80.2%) was found in Berkat
El-Sabaa.
2. A survey of root-knot nematode Meloidogyne spp. was
conducted in five locations of four different governorates. Sadat
city, Kafer El-Zayat, Kom Hamada, Wadi El-Natron and Giza
were the highest percentage of nematode occurrence (100%),
while the highest population density achieved 660.00 larvae /
250 g soil in Giza.
123
Summary
3. Culture filtrates of nine Fusarium spp. isolates reduced mixed
population of Meloidogyne spp. (M. javanica and M. incognita)
egg hatching under laboratory conditions. The greatest egg
hatching inhibition was recorded by Berkat El-Sabaa isolate,
while the least inhibition was recorded by Sadat city isolate.
4. Culture filtrates of nine Rhizoctonia solani isolates inhibited
mixed population of Meloidogyne spp. egg hatching under
laboratory conditions. The greatest egg hatching reduction was
recorded by Tanta isolate, while the least reduction was recorded
by Shibin El-Kom isolate.
5. Culture filtrates of nine Fusarium spp. isolates were effective in
reducing mixed Meloidogyne spp. larvae mobility. The greatest
larvae immobility was achieved by Berkat El-Saba isolate, while
the least one due to Sadat city isolate.
6. Culture filtrates of nine R. solani isolates were effective in
reducing mixed Meloidogyne spp. larvae mobility. The greatest
larvae immobility was recorded by Tanta isolate, while the least
one was recorded by Shibin El-Kom isolate.
7. All tested culture filtrates of biocontrol agents reduced mixed
Meloidogyne spp. egg hatching. The best isolate was Bacillus
megaterium, while the least one was Trichoderma harzianum.
124
Summary
8. All tested culture filtrates of biocontrol agents reduced mixed
Meloidogyne spp. larvae mobility. The best isolate was
B. megaterium, while the least one was T. harzianum.
9. All tested biocontrol agents were effective against pathogenic
Fusarium spp. isolates and inhibited the fungal linear growth.
Trichoderma koningii revealed the most effective fungal
bioagents, while Bacillus megaterium was the most effective
bacteria under laboratory conditions.
10. All tested biocontrol agents were effective against pathogenic
Rhizoctonia solani isolates and inhibited the fungal linear
growth. Trichoderma koningii revealed the most effective fungal
bioagents, while Bacillus megaterium was the most effective
bacteria under laboratory conditions.
11. All tested concentrations of marjoram and thyme wastes reduced
egg hatching of mixed Meloidogyne spp. under laboratory
conditions. The highest reduction was observed by 1/10
concentrate of the two wastes.
12. Different concentrations of marjoram and thyme wastes reduced
larvae mobility of mixed Meloidogyne spp. under laboratory
conditions. The highest reduction was observed by 1/10
concentrate of the two wastes.
125
Summary
13. Different concentrations of marjoram waste were effective
against pathogenic Fusarium spp. isolates and inhibited the
fungal linear growth under laboratory conditions. The highest
percentage of reduction (87.77%) observed by 1/10 concentrate,
while the least one (34.44%) observed by 1/100 concentrate.
14. Different concentrations of thyme waste were effective against
pathogenic Fusarium spp. isolates and inhibited the fungal linear
growth under laboratory conditions. The highest percentage of
reduction (87.77%) observed by 1/10 concentrate, while the least
one (24.44%) observed by 1/100 concentrate.
15. All tested concentrations of marjoram waste reduced the linear
growth of Rhizoctonia solani isolates under lab conditions. The
concentrate 1/10 gave the best reduction (84.44%), while the
concentrate 1/100 gave the least reduction (31.11%).
16. All tested concentrations of thyme waste reduced the linear
growth of Rhizoctonia solani isolates under lab conditions. The
concentrate 1/10 gave the best reduction (86.66%), while the
concentrate 1/100 gave the least reduction (23.33%).
17. Inoculation of root-knot nematode mixed population of
Meloidogyne spp. alone or with Rhizoctonia solani to the soil
infested with Fusarium oxysporum significantly increased
Fusarium wilt disease incidence under greenhouse conditions.
126
Summary
18. Inoculation of root-knot nematode mixed Meloidogyne spp. alone
or with F. oxysporum to the soil infested with R. solani increased
black scurf and stem canker disease incidence in pots.
19. Inoculation of F. oxysporum alone or with R. solani reduced
reproduction factor and all related parameters of mixed
Meloidogyne spp. in pots.
20. Interaction between root-knot nematode, R. solani and
F. oxysporum was more effective in reducing potato plants
growth parameter compared with any individual pathogen.
21. Under greenhouse conditions; application of Trichoderma
koningii and Bacillus megaterium alone or in combination seven
days earlier than soil infestation with F. oxysporum significantly
reduced Fusarium wilt disease incidence.
22. Under greenhouse conditions; application of Trichoderma
koningii and Bacillus megaterium alone or in combination seven
days earlier than soil infestation with R. solani significantly
reduced black scurf and stem canker disease incidence.
23. Application of the biocontrol agents T. koningii and Bacillus
megaterium alone or in combination significantly reduced
reproduction factor and all related parameters of mixed
Meloidogyne spp. in pots.
24. Biocontrol agent applications to the soil significantly improved
plant growth parameters.
127
Summary
25. Application of the endophyte Fusarium oxysporum one week
before soil infestation with F. oxysporum significantly reduced
Fusarium wilt disease incidence.
26. Application of the endophyte Fusarium oxysporum one week
before soil infestation with R. solani significantly reduced black
scurf and stem canker disease incidence.
27. The endophyte F. oxysporum significantly reduced reproduction
factor and nematode parameters on potato plants.
28. The endophyte F. oxysporum significantly improved plant
growth parameters.
29. Marjoram and thyme wastes were effective in reducing Fusarium
wilt when they were applied to the soil at the time of planting.
30. Marjoram and thyme wastes were effective in reducing black
scurf and stem canker by application to the soil at the time of
planting.
31. Application of marjoram and thyme wastes at the same time of
planting significantly inhibited the reproduction factor and
nematode parameters.
32. Application of marjoram and thyme wastes to the soil
significantly increased plant growth parameters.