الفهرس 
INNATE IMMUNITYOnly 14 pages are availabe for public view
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Abstract
In this study seven antimicrobial peptide genes [Tracheal Antimicrobial Peptide (TAP); Bovine Neutrophil β-Defensins (BNBD4, BNBD5, BNBD10 and BNBD11); Cathelicidin 4 (cath4) and Cysteine and Glycine Rich Protein (CSRP3)] were investigated in Egyptian river buffalo. The primers for these antimicrobial peptide-genes were designed using known cDNA sequences of Bos taurus published in database. Amplification of the above genes using Egyptian buffalo cDNA of different tissues (lung, mammary gland, ovary, colon, testis, tongue, muscle, intestine, trachea and liver) in addition to blood was performed. The PCR products of the target organs were one way sequenced. Bu-TAP cDNA, Bu-BNBD4 cDNA, Bu-BNBD5 cDNA, Bu-BNBD10 cDNA, Bu-BNBD11 cDNA, Bu-cath4 cDNA and Bu-CSRP3 cDNA sequences were deposited in the Genbank and were assigned accession numbers AB299970, AB299981, GQ231526, GQ231527, GQ231528, GQ354416 and GQ231523, respectively. BNBD5, BNBD10, BNBD11 and CSRP3 genes have not been sequenced before in buffalo.