الفهرس 
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Abstract
This study was done to evaluate the effect of maternal ethanol injection on the development of some major salivary glands of CD-I mice.
30 adult female (CD-I) mice and 15 adult male (CD-I) mice of average weight (25-30) gm were used in this study. The animals were housed in separate cages at the Faculty of Dental Medicine, Minia University, under the optimal experimental conditions.
I-Control group formed of 10 females and 5 males, neonates were divided into 2 subgroups:
I-Subgroup A considered as control group at birth (n=5). 2-Subgroup B considered as control at the day 21 after birth (n=5).
Il-Experimental group formed of 20 females and 10 males, neonates were divided into 2 subgroups:
I-Subgroup A considered as experimental group at birth (n=10).
2-Subgroup B considered as experimental group at the day 21 after birth (n=10).
For breeding, each two mature females were housed with one male over night in separate cages, from 5 pm to 9 am (day 0 of pregnancy). The next day was considered day I of pregnancy which was inspected by examination of the vagina (vaginal plug).
Neonate mice in subgroup A of control and experimental groups were sacrificed; heads were decapitated, fixed in buffered formalin solution, followed by dehydration, and embedding in paraffin. Coronal histological sections in heads of neonates were obtained of about 6 microns in thickness.
Mice in subgroup B of control and experimental groups were sacrificed.
Decalcification of specimens that contain bone were done first. Previous steps were repeated. All samples were processed for histological, immunohistochemical staining and image analysis for caspase 3 immunopositivity