الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The studies presented in this thesis include novel analytical methods based on spectrofluorimetric techniques for determination of some organic compounds used in pharmaceutical preparations. The thesis is divided to three chapters.
Chapter 1
This chapter includes general introduction of spectrofluorimetric activity of lanthanide elements and the parameters affects on these phenomena. Also, the chapter involved the review of literature for previous methods of metoclopramide and hydrochlorothiazide drugs determination.
Chapter 2
In this chapter a new, simple and accurate spectrofluorimetric method for the determination of metoclopramide hydrochloride (MCP) was developed. The metoclopramide hydrochloride can remarkably enhance the luminescence intensity of the Tb3+ ion doped in PMMA matrix at λex=360 nm in methanol at pH 6.9. The intensity of the emission band at 545 nm of Tb3+ ion doped in PMMA matrix is increased due to the energy transfer from metoclopramide hydrochloride to Tb3+ in the excited stated. The effect of different parameters, e.g., pH, temperature, Tb3+ concentration, foreign ions that control the fluorescence intensity of the produced ion associate was critically investigated. The calibration curve of the emission intensity at 545 nm shows linear response of metoclopramide over a concentration range of 5×10−5–5.0×10−8 M with detection limit of 8.7×10−10 M. The method was used successfully for the determination of metoclopramide in pharmaceutical preparations and human serum.
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Chapter 3
This chapter reports on a facile technique combined with a simple, sensitive and selective spectrofluorimetric method for the determination of hydrochlorothiazide (HCT). In methanol, at pH 8.3 and λex = 340, hydrochlorothiazide can remarkably enhance the luminescence intensity of the Eu3+ ion doped in polymethylmethacrylate polymer (PMMA) matrix. This could be due to the energy transfer from hydrochlorothiazide to Eu3+ in the excited stated. At the optimized experimental conditions, the enhancement of the characteristic emission band at 617nm of Eu3+ ion doped PMMA is directly proportional to the concentration of hydrochlorothiazide with a dynamic range of 5 ×10-8 – 1.0 ×10-5 mol L−1 and detection limit of 8.0 ×10−9 mol L−1. Application of the suggested method was successfully applied to the determination of hydrochlorothiazide in pharmaceutical preparations and human serum samples, with high percentage of recovery, good accuracy and precision.