الفهرس 
Avian influnza in humanOnly 14 pages are availabe for public view
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Abstract
Avian influenza has socio-economic importance all over the world. After the evolution of H5N1 in Hong Kong 1996 and spread in Asia, Europe, Africa with interspecies transmission and increasing human deaths threatening the world with a global pandemic. Emerging of Highly Pathogenic Avian influenza H5N1) in Egypt causes severe losses due to high mortality or culling of poultry. Although the promoters of endemicity in Egypt have not yet been clarified, silently infected free ranging ducks and geese as well as mixed species backyard holdings are suspected to play essential role in spread of the disease as they are difficult to be controlled. The rapid and accurate diagnosis of Avian influenza (H5N1) in poultry as well as gene sequencing of HA and PB2 genes are considered one of the most important tools used for the controlling the disease. The aims of this work are Collection of samples from different bird species from poultry farms and Screening of AIVs in the collected samples by Real Time PCR, Isolation of the positive samples in SPF chicken eggs for virus propagation, Characterization and phylogenetic analysis of the isolated viruses through sequencing of HA and PB2 genes. In this study sixteen samples from chicken, are submitted to CLQP in March 2011, and they are examined by real time PCR for M , H5and PB2 genes by pcr of AI and the results reveled all samples were positive for M and H5 genes. Isolation of sixteen samples through inoculation on ECE via allantoic cavity and all samples grew on the eggs and gave positive HA activity.