الفهرس | Only 14 pages are availabe for public view |
Abstract Twenty infected plant samples showing root rot symptoms (five potato tubers, five fbur tomato fruits, one bean plant and five radishes) were collected from different Ept and obtained from the Faculty of Agriculture, Alexandria University. The athogens were isolated on Sabaroud Dexstrose Agar (SDA) media, then the isolated re checked for their ability to cause root rot disease according to Kochs postulates. zhs positive pathogens were checked for their ability to grow on potato dextrose PDA), then identified using conventional and molecular diagnostic techniques. The :Dnal identification methods used in this study were the macroscopic and microscopic rzation, and protein profile of all samples are carried out too. The molecular diagnostic techniques applied in this study were the amplification of -cA sequences using universal primer, which followed by DNA sequencing. Then nr ecies specific primers with the most common identified samples. The fungi isolated zfected plants and proved to have the ability to cause root rot were characterized as *h:s: different isolates of the following fungi; Fusarium solani, Fusarium sp., *rhora sp, Fythium splendens. and Rhizoctonia solani which cause root rot disease. |