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Abstract A total of 35 extracted human premolars were used in this study. After extraction, the teeth were thoroughly cleaned from blood and any soft tissue, and stored in distilled water. Each tooth was sectioned mesiodistally into two halves down to the root after which halves were embedded in auto-cure acrylic resin blocks, maintaining a record of complementary halves. Teeth were divided into 5 main groups according to the dentin conditioner regimen; ranging from none, to that of NaOCl, or EDTA, or EDTA followed by NaOCl, or NaOCl followed by EDTA. Upon sectioning, halves were attributed to two subgroups according to the adhesive system used; whether Adper™ SE Plus or P90 System Adhesive, and their corresponding resin composites Filtek™ Z250 and Filtek™ P90, respectively. Two millimeter diameter resin composite specimens were attached to the dentin substrate via adhesive system, after which the whole assembly was stored in distilled water for 24 hours until shear bond strength testing. A total of seven specimens were tested for each subgroup. Shear bond strength testing was performed at a crosshead speed of 0.5mm/min, using a universal testing machine. Shear bond strength values, recorded in MPa, were tabulated and statistically analyzed, through which results of this study were documented and reasoned. The results of this study showed that Adper™ SE Plus as a methacrylate based adhesive recorded higher shear bond strength values than its counterpart for silorane-based composites. In contrast, the different dentin conditioners used caused a numerical increase in shear Summary & Conclusions 101 bond strength values, when compared to control receiving no prior conditioning. Conditioning performed via EDTA-NaOCl sequence exceeded that of NaOCl-EDTA, NaOCl, EDTA, and control, in that order. The highest mean shear bond strength was registered by subgroup Adper™ SE Plus following NaOCl and EDTA, while the lowest was recorded by P90 System Adhesive, surprisingly, that following NaOCl and EDTA. Conclusions: Within the limitations of this investigation, the following conclusions can be drawn: 1. The use of NaOCl, EDTA, and their combination as dentin conditioners had a positive effect on resin-dentin shear bond strength. 2. The methacrylate-based system was more positively affected by the different dentin conditioners used, compared to the silorane-based system, which was confined by its inherent strength; except for EDTA followed by NaOCl which seems to empower the silorane-based system. 3. The effect of NaOCl in increasing the bond strength was more evident when preceding EDTA, in case of methacrylatebased adhesive and the opposite was true in case of its counterpart for silorane-based composite. Summary & Conclusions 102 4. Resin-dentin shear bond strength of methacrylate-based system was consistently greater than that of silorane-based system, both with and without the investigated dentin conditioners. |