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العنوان
Effect Of Dietary Supplementation Of Some Probiotics And Prebiotics On Performance And Immunological Status In Japanese Quails =
المؤلف
Mohamed, Walaa Ahmed Mahdy
هيئة الاعداد
مشرف / محمداسماعيل القطشة
مشرف / عصام يوسف اسماعيل
مناقش / السيد محمد حجازى
مناقش / مسعد عبد الخالق سلطان
باحث / ولاء أحمد مهدى محمد
الموضوع
Nutriton.
تاريخ النشر
2012.
عدد الصفحات
116 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
25/11/2012
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - التغذية والتغذية الاكلينيكية
الفهرس
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Abstract

This work was conducted to study the effect of dietary supplementation of probiotics, (Protexin® and AM Phi- Bact) and / or prebiotic (AGFLO® and Alphamune) on growth, immune response, some blood parameters and carcass traits of Japanese quail. This study depends on recording the results concerning body weight development, body gain, feed conversion ratio, relative growth rate, measuring some blood parameters, immune response and carcass traits of Japanese quail during the growing period. A total number of 360 one day-old chicks were used. The chicks were randomly allocated into 9 equal groups (40 per each) of mixed sex. The J. quail groups were fed as follows:
Chicks of group 1 were fed on the basal diet without any supplementations as the control group. While chicks of groups 2, 3, 4 and 5 were fed on the basal diet supplemented with Protexin®, AMPhi- Bact ,AGFLO® and Alphamune at the rate of 0.1, 0.325, 0.5 and 0.5 g/kg diet respectively. While quail chicks of groups 6, 7, 8 and 9 were fed basal diet supplemented with Protexin® plus AGFLO® , Protexin® plus Alphamune, AM Phi- Bact plus AGFLO® and AM Phi- Bact plus Alphamune respectively.,
The obtained results can be summarized as follows:
1- At the end of the experiment the highest body weight was observed by quail groups No. 6 which was fed on the basal diet supplemented with probiotic (containing entrococcus) plus AGFLO followed by group No. 5 which was fed on the basal diet supplemented with Alphamune product based on mannan oligosaccharide (MOS). While the lowest body weight was obtained by quail chick group No.1 which fed on the basal diet without supplementation.
2- It was observed that there was insignificant increase in BWG in quails of all experimental groups when compared with control group.
3- Concerning the last week of experiment, groups 2, 5, 8, 9, 6 and 7 showed significant increase in RGR when compared with control group. While other treated groups 2 and 4 showed numerical increase in RGR when compared with control group. Regarding total RGR insignificant increase in RGR was detected in groups 4, 7, 3, 5 and 6 while numerical decrease was found in other groups when compared with control group.
4- Total feed intake was clearly reduced due to combination between both probiotics with AGFLO and Alphamune prebiotic (groups 6 – 9) through the experimental period when compared with the control.
5- It was observed that probiotic supplementation (groups 2 – 3) non significantly effect on average FCR throughout the whole experimental period when compared with the control, while prebiotic supplementation (groups 4 -5) revealed non significantly improved FCR when compared with the control. On the other hand, supplementation of probiotics plus prebiotics (groups 6 -9) significantly improved average FCR throughout the whole experimental period when compared with the control.
6- The ratio between weight gain and protein intake increased significantly in probiotics and prebiotics groups than control group in the first, 2nd and 3rd week of experiment. It was observed that combination between probiotics and prebiotics (groups
6 -9) significantly improved PER when compared with the control in the last 2 weeks of the experiment.
7- An improvement in both phagocytic activity and phagocytic index in prebiotics supplemented groups. Numerical increase in phagocytic activity was noticed in groups 4 and 5 which were fed BD supplemented with (AGFLO and Alphamune). While significant increase in phagocytic index was detected in groups 6, 3. 4 and 8 when compared to the control group.
8- A little effect of probiotic and / or prebiotic supplementation on percentage of different elements of WBCs. The noticeable changes due to prebiotic supplementation were the insignificant increase of Lymphocytes in groups 5 and 8 which fed BD supplemented with Alphamune and AM Phi- Bact plus AGFLO when compared to the control group.
9- There was a significant increase in HI titer to ND a day 24 in groups 6, 7 and 8 which fed BD supplemented with Protexin plus AGFLO , Protexin plus Alphamune AM Phi- Bact plus AGFLO when compared with those of control group. Other groups showed insignificant increase in HI titer when compared to control group. However at day 33 and 42 there was a significant increase in HI titer to ND in groups fed BD supplemented with probiotics plus prebiotics except those of group 6.
10- Combination between probiotics and prebiotics (groups 6 -9) significant improved in total protein, serum albumin and globulin when compared with the control. It was observed that combination between probiotics and prebiotics (groups 6 -9) showed numerical decrease on serum GOT when compared with the control. On other hand combination between probiotics and prebiotics (groups 6 -9) showed significant (P≥0.05) difference on serum GPT when compared with the control.
11- It was observed that quail groups fed diet containing combination between probiotics and prebiotics (groups 6 -9) showed numerical decrease in serum cholesterol when compared with the control group. Also, combination between probiotics and prebiotics (groups 6 -9) showed significant difference on serum triglyceride levels when compared with the control. There was insignificant difference in serum triglyceride levels between other treatment groups (2-4) when compared with control groups.
12- There was no significant difference between supplemented and control groups in dressing % and organ weight percent to carcass weight of liver, heart, spleen, bursa, fat, gizzard and proventriculus.