الفهرس 
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Abstract
The present investigation is to study the effect of orally administered sildenafil,
ginseng or both on the reproductive functions of female mice (immature and mature female
mice).
The following items were investigated:
1. The effect of giving sildenafil, ginseng or both orally on the reproductive function
of immature female mice (body weights, ovarian and uterine weights, hormonal
assay and number of small medium and large sized follicles).
2. The effect of giving sildenafil, ginseng or both orally on the ovarian function of
mature female mice (gonadosomatic index, hormonal assay and number of small
medium and large sized follicles).
3. The effect of giving sildenafil, ginseng or both orally on the uterine muscle tone
during the estrus phase of estrous cycle in mature female mice.
Three experiments were carried out
The first experiment:
UAim:
The aim of this experiment is to evaluate the effect of oral administration of
sildenafil, ginseng or both on the reproductive function of immature female mice.
UExperimental work:
Fourty immature female mice were used. This group was further subdivided into 5
subgroups (8 mice each) namely group Ic, group If, group Is, group Ig and group Isg.
Mice in group Ic were administered a daily dose of 0.2 ml saline orally for five
successive days and this group served as control. In the group If, mice received daily oral
dose of 0.2 ml saline for five successive days then were injected intraperitoneally (I.P) with
0.1 ml of saline containing 5 I.U. of FSH (Fostimon) followed 48 hours by an I.P. injection
of 5 I.U. of LH (Pregnil) to induce super ovulation. Group Is was treated as group If and
instead of saline they received oral dose of sildenafil citrate (10mg/kg body wt.) for five
successive days by stomach tube. In the group Ig mice were treated as group If and
received a dose of ginseng (10mg/kg body wt.) orally for five successive days. Mice in
group Isg were treated as group If and instead of saline administered sildenafil citrate
(10mg/kg body wt.) and ginseng (10mg/kg body wt.) for five successive days orally.
Mice in all groups were weighed then exsanguinated 12 hours after the last
treatment dose and blood samples were collected and serum samples were separated then
used for estimation of serum estrogen and progesterone levels. Ovaries and uteri were
dissected out and weighed. Ovaries and uteri were processed for histological sectioning.
The number and size (μ m) of growing and large follicles were determined