الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
Lantana camara Linn., family verbenaceae had a
potent antifeedant activity. oonsequantly, it was important
to study the antifeedant activity of ~. camara and to
isolate, purify and identify the principal or principals
responsible tor this property.
Tile plant was extracted with solvents of increse ing
polarity (oold extract) and a phytochemioal screening was
made on the soluble-suocessive extracts, as well as dry
powder. it could be concluded that -L. oamara leaves contain
mainly sterols and/or triterpenes. free and combined
flavonoids. glycosides and/or carbohydrate, tannins,
saponins and sublimable SUbstances. Alkaloids and Nitrogenous
bases are absent. Pet. ether extract contains
mainly sterols and/or tri. terpenes.
Feeding detrrent screening tests of tile extracts
was made acoording te Butterworth (1971).The percent reduction
of feeding/control was calculated. The 5-th instar
nymphs of ~. gregaria were used. The screening showed
that pet. ether extract was the most potent followed by
ethanol 70 ~, then ether, aceton, chloroform and dis.
water (91.16, 85.23, 83.73, 70.21, 42.30 and 37.42
respectively) •
Dried powder was extracted with pet. ether in a
soxhlet extractor (Hot e2tract), the extract was examined
for its antifeedant properties. Feeding tests showed
95.69 % reduction of feeding/control. Therefore the pet.
ether extract was fractionated on a column of alumina,
using the solvent system pet. ether (FI); benzene (F2),
benzene mixed with chloroform in an increasing proportions
(20 to 50~) (,), chloroform (’4)’chloroform (F5) and
chloroform: methanol (95 : 5 %) (P6)·
Fraction ) was the most potent as antifeedant
(9).24 %). TLC examination of fraction 3 revealed the
presence of 2 spots (A and B) one of them was parallel to
lantadene A while the other one was parallel to Lantadene
B.
For better separation am isolation of the active
ingredients, fraction .3 was acetylated and the acetylated
product was intro~uced to preparative TLC chromatography,
each developed band was scraped and separately with
chloroform, and the acetyl group eli~ated, the two free
components A and B were solely examined for their antifeedant
properties. The results Obtained, showed that neither
component A (63.25 %) nor component B (40.96 %) reacned
the antifeedant activity of their mother mixture of fraction
3 (93.0) %). TLC technique of the two components of
’3 by visualization by U.V. 1ight lISS used to avoid any
chemical treatment which migb-t effect the stereochemical
structure of these oompoundswhich may effect their
biological properties.
Result s revealed that 1’3 and component Awere
active as antifeedants (92.77 and 87.61 % respectively).
Such results might support the deduction of some change
in stereochemical structure during acetylation and removal
of the acetyl group.
Although, Jf
has a lower antifeedant aoti vity but
it showed two new and unknown spot s on the TLC chromatogram.
These two spots were corresponding to the same two
spots in the original fraction. TLCseperation of F6 by
visuall zation Wlder U.V. Light revealed that fraction 6
is a mixture of two steroid sUbstances C and D respectively.
Canponents A, B, C and D were examined for their
purity by detemining their melting point, optical rotation.
elementary analysis, U.V., I.R. and 11.JI.R. Speotroscopic
analysis.
The obtained results of the t1l0 components It. and
B are almost in oompelete agreement 111tb. t bose Obtained
for the two authentio samples Lantadene A and Lantadene B.
The results obtained for the ’other two compou.n.dsC and D.
were almost identical with that reviewed befor for o1eanolic
acid and 22-B-hydroxy oleanonic acid respectively.
Different concentrations of componentAt 70, 35,
17.5 mg/100 ml. were t eated on ~. gregaria. Th.eobtained
results showedthat the concentration 35 mg/100 ml. EtCH
is the thresnold potent concentration 88 an1iifeedant.
Stock solutions of the componentsA, B, C and D
were prepared by dissolving 35 mgof each in 100 ml, ,
eth&nol. To study the biological effects different
solutions were used. Results revealed slight toxicity by
contact a nd the compounddid not exerl ant’ a.dverse biological
effect on the 6th instar Larvae of !. littoralis
when topically applied. The results revealed some toxio
effects by feeding and no oUler adverse biological effects
on the 6-th instar larvae or adults of -S. littoralis. It was evident that componentA produced somedegree of
feeding deterrence to §. littoralis.