الفهرس 
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Abstract
J. Introduction and literature survey
Family Myrtaceae comprises many genera and several species which are reported to have a variety of active constituents that have different biological uses. The present work includes phytochemical investigation and evaluation of some biological activities of C. viridiflorlls leaves.
2. Phytochemical screening.
A phytochemical screening of C. viridiflorus resulted In the presence of flavonoids, tannins, volatile oils, carbohydrates and/or glycosides, triterpenes and/or sterols and saponins. Also, it revealed the absence of alkaloids, anthraquinones and coumarins.
3. Study of lipoidal content of C. viridiflorus leaves
This part deals with the preparation of both unsaponifiable matter (llSM) and fatty acid methyl esters and their GLC analysis. This study showed that the percentages of hydrocarbons and sterols were (76.79 %) and (2.87%), respectively. It was found that n-nonane (11.94%) and n¬octadecane (10.97%) represented the major hydrocarbon while stigmasterol (1.31 %) represented the major sterol. Concerning the composition of fatty acid content it could be concluded that the saturated fatty acids (84.426 %) represented higher percentage than that of unsaturated ones (14.807 %). Arachidic (42.352 %) and palmitic (37.006%) acids represented the major identified saturated fatty acid while oleic acid (5.463 %) represented the major unsaturated fatty acid.
4. Investigation of essential oils of C. viridiflorus leaves and flowers
This part showed how essential oils distillated from C. viridiflorus leaves and flowers by hydro distillation and their GC\ MS analysis. This
study -;howed that Methyl eugenol (95.07%) in flowers, leaves represented the major component of the essential oil.
S. Investigation of phenolic constituents of C. viridiflorus leaves
Air dried powder of the leaves and stems of the plant under investigation (l500 gm) were subjected to exhaustive extraction with 80 % MeOH under reflux. After drying the extract under reduced pressure, the residue was defatted with pet. ether (60-80° C). The 2D-PC analysis proved that pet. ether extract is polyphenolic free, while polyphenols are concentrated in methanol soluble portion. Methanol soluble pOliion was subjected to fractionation on polyamide H20/MeOH mixtures. Final purification of isolated compounds was achieved using Sephadex LH-20 and cellulose columns with convenied solvent systems. Identification of isolated compounds based on chemi and physical methods including UV, Negative ESI-MS/MS, IH/13 NMR, HMBC. These studies led to isolation and identification of ei polY1-Jhenols as;
1- Gallic acid was isolated the frist time from C. viridiflorus identified before from the leaves of C. lanceolatus 2- Ellagic acid was isolated the frist time from C. viridiflorus identified before from the leaves of C. lanceolatus
3- 1,2: 3,4-bis(s)-hexahydroxy diphenoyl-,8-D-glucopyranose isolated for the frist time from genus Callistemon.
4- 2,3-0- hexahydroxydiphenoyl-(al,8)-glucopyranose is isol for frist time from genus Callistemon
5- Quercetin 3-0-a- L- glucuronopyranoside is isolated for time from genus Callistemon 6- Quercetin 3-0-,8- D- glucopyranoside was isolated once from the leaves of C. lanceolatus .