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Abstract Green algae and many microbial organisms like Trichoderma are rich with vital substances that have a lot of activities, including its ability to resist some pathogenic organisms, which threaten the life of the plant and human. This study was conducted on several marine green algae and some species of Trichoderma to study the effect of the extract of these organisms on the growth of some pathogenic species from bacteria and fungi and isolate the substance (s) responsible for the antimicrobial activity of the organism which showed the greater activity for inhibiting the growth of bacteria and fungi using several modern techniques as cloning and expression for the production of this substance (s) in the laboratory. To achieve this goal, some marine green algae were collected and extracts were made from them and their impact on the growth of some pathogens was studied. After that fatty acids content were isolated from Enteromorpha intestinalis. Also β-1, 3-endoglucanase was isolated from T. viride. Finally, cytological studies were conducted to study the effect of these two substances on a test plant monitoring the number of chromosomal aberrations during mitosis but these changes are statistically not significant. |