الفهرس 
Part I: General introductionOnly 14 pages are availabe for public view
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Abstract
The thesis is devoted to spectrophotometric, chromatographic and
electrophoretic analysis of antihypertensive drugs which has an
urgent need in the pharmaceutical field nowadays, as well as the
crucial importance of the analysis of enantiomers as almost most of
drugs in use are chiral. Four antihypertensive drugs namely
Amlodipine besytale (AML), Perindopril Erbumine (PER), Valsartan
(VAL) and Hydrochlorothiazide (HCT) have been determined via
this approach. The thesis comprises four parts:
Part I. General introduction
This part provides a general introduction about hypertension and an
analytical review for the analytical techniques used for the
determination of the studied drugs either in dosage forms or in
biological fluids.
Part II: This part consist of four sections Section (A): The suggested method based on measuring the
peak amplitude of the first derivative of the ratio spectra for the
simultaneous determination of amlodipine besylate and perindopril
erbumine, each peak amplitude was measured at the specified
wavelength. Section (B): This method develops a stability indicating ion pairing isocratic reversed phase HPLC method for the simultaneous
determination of binary mixture of AML besylate and PER erbumine
in presence of their degradation products. The efficient separation was achieved on Zorbax C18 analytical
column using mobile phase consisting of 0.05 M phosphate buffer:
acetonitrile in the proportion of (30/70, v/v) with the pH adjusted to
of 3.0 with orthophosphoric acid.
Section (C): TLC-densitometric method was developed for the
simultaneous determination of binary mixture of AML besylate and
PER erbumine. The efficient separation and identification were
achieved on utilizing n-butanol: water: acetic acid in the ratio
(4:5:1by volume) as a mobile phase.
Section (D): Development HPLC method simultaneous
determination of AML besylate, VAL and HCT in bulk powder,
laboratory prepared mixtures and its tablet dosage form.
Part III: This part consist of three sections Section (A): The suggested HPLC method based on separation of
S-PER in presence of its R-enantiomer using of β-cyclodextrin as a
chiral selector through ChiraDex chiral column. The efficient
separation and identification were achieved using acetonitrile and
0.05 M potassium dihydrogen phosphate solution with the pH
adjusted to of 3.0 with orthophosphoric acid in the proportion (45:55
v/v) as a mobile phase.
Section (B): The proposed CE method based on separation of
S-PER in presence of its R-enantiomer using Hydroxypropyl β-
cyclodextrin as a chiral selector. Different factors affecting
electrophoretic mobilities were studied such as buffer type, pH,
chiral selector, organic modifier and analytical voltage.
Summary and Conclusion