الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 125 |  |  |
| | | from | 125 | | |
Abstract
necessary inforwa~ions for ditte~cnt1atinr- between four virusla &ttecting cuourblta
1- Eskandarani squash ~a5 the only- hoat that revealed susceeptible to all tested virusea, narnel.y, AJN, ArldV, OW and SQ!.!V. SytnDtoc expression obtained on this host \”11 th the 4 viruses was differential only during the early stages of 1nfection (i. e., ten to 14 days after inQcttlatincr the coteledonary leaves). At that tioe, AWl caused pecu11ar spotty mosaic, AsMV induced a spotty moaaic disease also but the spots uere considerably smaller than those 01’ AMV; CMY initiated ord1nary mot~nG, and SqMV incited mottl1qg assoc1ated w1th narrouing of 1nterveinal angles and deepenning 01’ leaf 1nc1sa10ns.”
2- Alfalfa mosaic v1rus 1n1’ected 15 plant svecies out of
18 mechanically 1noculated ones, v/l1ereas AsL”V, OJlV and SqMV 1n1’ected, ¥espect1vely, 17, 15 and 1 plan_ spec1es ,out of 19 1nfected ones.
3- On the bas1s 01’ host range and symptomatology stud1es ’
it was concluded that tor diagnoa18 ot these virus ••
and 41tterent1at18n between th • the 1nolusion of the,
! {f •
tollowing 6 plant-species in mechanical transmission
tests is the most reliaDle means.Asclepias (urassaviea, Datura metel;Eskandar~ni squash,Niootiana rustic!, Giza 2 Broad bean and Azmyrlie Cowpea.
tested; it ~ithstQQd heating for 10 winutes to ~ore
than 90 C. 8QMV showed to be tbe second tbePi:io-stable
virus; its tber:•.ial inactivation point as revc8.1ed by te”sting on ~sl:anc..arani sQuacb vms 750C. ’.;:l1e tl1erl:ial
inactivation point of AI;’! licd. betvlcen 65 an:!. 70 c. C:.-:V
Vias inactivate:!. b:l beatine for 10 tiinut8S’ at G50C but
not at 60 C.
6- The dilu ti on end.-l)Qint of .:.\J.-:V , Asi,::V , G.,:V and 8 Qj,:V \7ere 10-4,10-6,10-5 and 10-5, resIlectivel:l.
7- The 10nC;evi ty in vi tl’O Clt l’OOU teq)el”ature of tbe viruses
under investiGation uas as follovs : less than 5 d~TS for AMV, more than 98 days fOl’” Af!¥Il, less tban 4 d8;\,Ts for
and hydrocen pel”’oxide on virua acti vi ty l’evealed tbo t all viruses \7ere completel:’l inbi bi teu. \”lhen infectious sap was tl’e~tea. for 60 1111 nu tes \’/i tb 80 % e tlDl101
a) AMV, CMY and S~v.v were treated with 20 or 40 ~ phenol,
b) AMY ith 75 % alcohol, 0.5 or 1 % formaldehyde,
c) GIN fa th 1 % formaldehyde, and d) SgMV with 10 % phenol or 2 5~ hydrogen Deroxide.
Within the ranGe of concentrations used, phenol revealed tti’ be the most effective round in inactivating viruses in vitro.
9- lio evidence that AMV, ASMV, CMV and SqMV are seed-qorne
in Eskandarani squash was bl’ought about. Seed Q.uali ty, hOl’llever, ~as clearly affected by virus infections. S~, CWI and AMY substantially reduced the ~eight, specific weiGht and :percentage of gerl::iination of skandarnni sQ.uash s2eds. AsMV slightly reduced the weight and germination percentage of seeds, whereas it slightly increased the seeds’sDecific weight.