الفهرس 
Pharmacology of cisplatinOnly 14 pages are availabe for public view
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Abstract
Cisplatin is an important anticancer agent. Its chief dose-limiting adverse effect is nephrotoxicity. Pharmacological treatment of cisplatin nephrotoxicity is unsatisfactory. Stem cells have proven efficacy in the treatment of cisplatin nephrotoxicity in rats. Similarly, the conditioned medium derived from stem cells is also effective in the treatment of cisplatin nephrotoxicity in rats. The aim of this study was to compare the efficacy of either strategy using sensitive markers of acute kidney injury. In addition, comparison extended to both long-term benefits and hazards of both strategies, namely fibrosis and differentiation to unwanted cells. To induce cisplatin nephrotoxicity in rats, animals received a single dose of cisplatin (5 mg/kg). In the presence of suitable parallel control groups, rats were treated with either stem cells isolated from the bone marrow or the conditioned medium obtained from cultivation of stem cells. The evaluated biochemical parameters were serum creatinine, creatinine clearance, blood urea nitrogen, serum magnesium, and urinary kidney injury molecule levels. The evaluated pathological parameters were ordinary pathology by light microscope, tubular cell proliferation and apoptosis, total collagen and interstitial fibrosis, and detection of adipocytes in the kidney. The results of the study demonstrated that both stem cells and the conditioned medium were able to ameliorate renal tissue injury that was induced by cisplatin. Only in proliferation, stem cells were more potent in enhancing tubular epithelium proliferation but adipocytes were detected where stem cells were injected. This study suggests that as stem cells act via a paracrine mechanism, manipulation of the conditioned medium could provide similar benefits of those of the stem cells but may avoid the possible hazard of stem unwanted differentiation to adipocytes.