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Abstract Randomly, 160 fresh water fish samples ([80 samples from Nile tilapia (Oreochromis niloticus) and 80 samples from Nile catfish (Clarias gariepinus)] were collected from River Nile, earthen ponds and Mansouriya canal in Cairo, Giza, and Qalyobiya governorates, EGYPT. Some examined fish showed severe skin lesions, skin ulcers and peticheal hemorrhage. Morphological, biochemical and molecular identification of bacterial isolates revealed F. columnare and C. indologenes. Total incidence of Flavobacteria was 65.6%, with individual incidence of 70% in Nile catfish and 61.25% in Nile tilapia. Physicochemical parameters of examined water samples favor disease development. |