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العنوان
Mollecullar Characteriizatiion of the associiated antiimiicrobiiall genes of Sallmonelllla Serovars recovered from poulltry /
المؤلف
Mohamed, Reham Ahmed Hosny.
هيئة الاعداد
باحث / ريهام أحمد حسنى محمد
مشرف / كاميليا محمود عثمان
مشرف / وفاء محمد محمد حسن
الموضوع
Salmonellae. Virulence genetics. Antibiotic resistance in microorganisms.
تاريخ النشر
2013.
عدد الصفحات
332 Leaves :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2013
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
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Abstract

Out of 71 eggs collected from different sources and different origins, the incidence of
Salmonellae was in a percentage of 1.4%. Virulotyping analysis by PCR for 11 virulence
genes (invA,avrA,mgtC,ssaQ,siiD,sopB,bcfC ,spvC, sodC1 ,sopE1 and gipA) were done on
Egg and Human serovars (14), Highest virulence genes recorded were : invA and siiD genes
that were detected in a percentage of 100% in each and avrA, ssaQ, mgtC, siiD and sopB that
were detected in a percentage of 89 % and 100% respectively. gipA was absent in each. All 84
serovars showed different resistance pattern for 13 antibiotics (Ampicillin , Amoxicillin ,
Chloramphenicol , Streptomycin , Tetracycline , Trimethoprim , Ciprofloxacin , Colistin
Sulphate , Gentamycin ,Lincomycin , Nalidixic , Norfloxacin and Neomycin). All serovars in
this study showed highest resistance to Lincomycin (L), Streptomycin(S) and
Chloramphenicol(C). Analysis of antibiotic resistance genes for all 84 serovars by PCR
showed that Class 1 integron was detected only in Human,Turkey, Egg, Duck and Chicken
serovars in a percentage of 90%, 71%, 56% , 26% and 11%. thdF gene of Left Junction of
SGI1 was recorded in Turkey, Duck, Egg and Human serovars in a percentage of 80%, 56%,
40% and 33% respectively. yidY gene of right Junction of SGI1 was detected in Chicken ,
Duck , Turkey ,Egg and Human serovars in a percentage of 100% , 67% ,60% ,60% and 45%.
int 2 gene of right Junction of SGI1 was present only in Turkey and Human serovars in a
percentage of 40% and 11% respectively. aadA2 gene was detected only in Human serovars
in a percentage of 44%. floR gene was present in Chicken, Turkey, Duck, Egg and Human
serovars in a percentage of 100%, 60%, 22%, 20%, 11% respectively.