الفهرس 
Chemical review of genus HeimiaOnly 14 pages are availabe for public view
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Abstract
Investigation of phenolic leaf extract of Heimia myrtifolia (Lythraceae):
Pharmacological properties (stimulation of mineralization of SaOS-2
osteosarcoma cells) and identification of polyphenols
Evaluation of the activity of an aqueous alcoholic extract obtained from the leaves of
Heimia myrtifolia (Lythraceae) by determining its stimulating effect on two human
osteoblastic cell lines HOS58 and SaOS-2 proved possible prevention and treatment
of osteoporosis. In addition, the extract was found to increase significantly the
mineralization of cultivated human bone cell SaOS-2, whereby a strong dose
dependent increase was observed. On the other hand, a phytochemical investigation
of the extract confirmed that H. myrtifolia is capable of synthesizing and
accumulating appreciable amounts of several phenolics, thus leading to the isolation
and characterization of sixteen of these constituents. Among these isolates the new
natural product, 1,6-di-O-dehydrotrigalloyl-β-D-4C1-glucopyranose and the rare
natural product (secondly reported) 5,7,4’-trihydroxy-3-methoxyflavanone;
dihydrokaempferol-3-O-methyl ether were fully identified. All structures were
elucidated on the basis of conventional methods of analysis and confirmed by
ESI/MS and, 1H and 13C-NMR analysis.
Keywords:Heimia myrtifolia, phenolics, osteoporosis, 1,6-di-O-dehydrotrigalloyl-β-D-
4C1-glucopyranose
Published as original research article in the Journal of Drug Discoversei and
Therapeutics (Japanese Journal), 2010; 4(5): 341-348.
Enhancement Activity of Calcium Deposition of SaOS-2 Human Osteosarcoma
Cells by Phenolic Leaf Extract of Heimia myrtifolia
Evaluation of the activity of an aqueous methanolic extract obtained from the leaves
of Heimia myrtifolia (Lythraceae) by determining its stimulating effect on the two
human osteoblastic cell lines HOS58 and SaOS-2 proved possible prevention and
treatment of osteoporosis. In addition, the extract was found to increase significantly
the mineralization of cultivated human bone cell SaOS-2, whereby a strong dose
dependent increase was observed.
On the other hand, a phytochemical investigation of the extract confirmed that
Heimia myrtifolia is capable of synthesizing and accumulating appreciable amounts
of several phenolics, thus leading to the isolation and characterization of sixteen of
these constituents. Among these isolates the new natural product, 1,6-di-Odehydrotrigalloyl-
β-D-4C1-glucopyranose was fully identified. All structures were
elucidated on the basis of conventional analytical methods and confirmed by
ESI/MS, 1H and 13C-NMR analysis.
Poster presented at 13th Conference for the Pan Arabian Conference for the
Colleges of Pharmacy of the Assembly of Arab Universities (AARU)Hilton
Dream Land Hotel, 6th October City, Cairo, Egypt. May, 2010