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Abstract A total of266 collected rectal swabs (141) from cattle calves and 125 from buffalo calves) were examined bacteriologically. E. coli was present in 20 (14.2%) and 29 (23.2%) respectively. -Bacteriological examination of266 diarrhoeic calves with different ages from birth upto 6 months old revealed that E. coli infection was increased during the first few weeks of life and decreased by aging. -All 49 E. coli isolates were tested serologically. E. coli 0157 was present in 2 isolates in an incidance of 4.0%. -Studying the virulence factors of E. coli revealed that: -10.2 (5/49) were a - Hly producing E. coli. -4.0 % (2/49) were E-Hly producing E. coli, 1 of the two was serotyped E. coli 0157. -Using peR to detect the presence of STa gene in three groups ofE. coli revealed that 100.0% (2/2) E. coli 0157 group were negative, 16.6% (116) of the haemolytic group was positive and 66.6% (4/6) of random E. coli group were positive. -Two E. coli isolates were positive by 0157 antisera and shared CPE in Vero cells indicating Verotoxigenic E. coli 0157 (0157 VTEC). -Two E. coli isolates were negative by 0157 antisera and shared CPE in Vero cells indicating Verotoxigenic non-0157 (Non-0157 VTEC). -One E. coli 0157 was positive for tube agglutination test indicating E. coli 0157: H7. -All of the E-Hly producing isolates were Verotoxin producing E. coli. |