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العنوان
Physiological and ecological studies on the responses of some melanin producing fungi to certain toxicants /
المؤلف
Khattab, Khattab Afifi Fahmi Ali.
هيئة الاعداد
باحث / KHATTAB AFIFI FAHMI ALI KHATTAB
مشرف / Mohammed Medhat Gharib
مشرف / Sabha Mahmoud El –Sabbagh
مناقش / Sabha Mahmoud El –Sabbagh
الموضوع
Sanitary microbiology.
تاريخ النشر
2013 .
عدد الصفحات
187 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/10/2013
مكان الإجازة
جامعة المنوفية - كلية العلوم - Botany department.
الفهرس
Only 14 pages are availabe for public view

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from 187

Abstract

Melanin are found in most living organisms, dark or dark brown pigments, it is polymer and have different character and uses. It protect living organisms from UV radiation and protect microorganisms from outer virulence by other microorganisms and protect from anther un suitable environment. This studies included. 1-Isolation and identification of fungi that produced melanin pigment .The isolate no. 1 and 2 were identified as Drecheslera rostorata and Cladosporium herbarium. The studies includes extraction, purification of the melanin pigment. The chemical and physical characters of melanin pigment of two fungi illustrate that this pigment don’t soluble in organic solvent but it soluble in NaOH at 100 0C for two hr., and also give positive reaction when react with phenolic compound and brown precipitation with FeCl3. 2- The experimentation was continued to find out the most optimal conditions. The most suitable environment conditions for melanin
production was studied growth pH 6 was suitable for two fungi in dark
in Czapkś Dox. media . Glucose and sodium nitrate were a suitable
carbon sources and nitrogen sources.
3- The effect of metalloids( K2CrO4, NaSe2O3, CoCl and NaTe2O3) on
growth of fungi that produced melanin showed that that the growth of the
two fungi decreased with increasing the concentration of metalloids.
summary
4-The effect of UV-B radiation on fungi producing melanin resulted in
the production of two types from Drecheslera rostorata and named as
the following ( Albino, Black).
5- Determination of metalloid concentration inside the cell it was found
that the concentration of metalloids increased in albino than in wild and
in black.
6- Determination of carbohydrates , total lipids, amino acids and total
protein it was found that this compound decrease with presence of
metalloids and production of tyrosine amino acid in control black and
treated black with metalloids were more than its production in control
albino and treated albino with metalloids.