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Abstract Melanin are found in most living organisms, dark or dark brown pigments, it is polymer and have different character and uses. It protect living organisms from UV radiation and protect microorganisms from outer virulence by other microorganisms and protect from anther un suitable environment. This studies included. 1-Isolation and identification of fungi that produced melanin pigment .The isolate no. 1 and 2 were identified as Drecheslera rostorata and Cladosporium herbarium. The studies includes extraction, purification of the melanin pigment. The chemical and physical characters of melanin pigment of two fungi illustrate that this pigment don’t soluble in organic solvent but it soluble in NaOH at 100 0C for two hr., and also give positive reaction when react with phenolic compound and brown precipitation with FeCl3. 2- The experimentation was continued to find out the most optimal conditions. The most suitable environment conditions for melanin production was studied growth pH 6 was suitable for two fungi in dark in Czapkś Dox. media . Glucose and sodium nitrate were a suitable carbon sources and nitrogen sources. 3- The effect of metalloids( K2CrO4, NaSe2O3, CoCl and NaTe2O3) on growth of fungi that produced melanin showed that that the growth of the two fungi decreased with increasing the concentration of metalloids. summary 4-The effect of UV-B radiation on fungi producing melanin resulted in the production of two types from Drecheslera rostorata and named as the following ( Albino, Black). 5- Determination of metalloid concentration inside the cell it was found that the concentration of metalloids increased in albino than in wild and in black. 6- Determination of carbohydrates , total lipids, amino acids and total protein it was found that this compound decrease with presence of metalloids and production of tyrosine amino acid in control black and treated black with metalloids were more than its production in control albino and treated albino with metalloids. |