الفهرس 
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Abstract
The role of Adeno-virus as an etiologic agent of bovine respiratory and enteric diseases has much attention in recent years, and the Adeno-virus
are responsible for producing great losses to the livestock industry in many ways.
In the present study aiming to prepare and evaluate inactivated monovalent freeze dried Adeno-virus vaccine for control the spread of this viral agent in Egypt, and to assist in saving animal industry in Egypt.
The susceptibility of different cell cultures (BK, MDBK, VERO, CER) for cultivation of Adeno-virus was studied to choose the best cells for
vaccine production. MDBK was the culture of choice as it produces high titre and was in preparation.
Binary ethyleneimine was used for the inactivation of Adeno-virus, different concentration of Binary ethyleneirnine at different times were made to detect the suitable concentration of BEI at a proper time for complete inactivation of Adeno-virus. Trials of virus re-isolation indicated that the Adeno-virus was not isolated from the buffy coat, nasal, conjunctival and rectal swabs of either vaccinated or contact control calves (control non infected calves) throughout 14 days post vaccination. Results indicated that the Adeno-virus was reisolated from vaccinated challenged calves for 3 and 4 days post challenge from the vaccinated groups by the monovalent inactivated vaccine. But in control infected calves, the virus was isolated from 1-12 days post infection, the Adeno-virus was identified by the use of specific hyperimmune serum by using SN test.
The mean total leukocytic count showed moderate decrease as the result of vaccination from the 3rd to the 5th day post vaccination which accompanied by moderate decrease of lymphocytes percentages from the 3rd to bth days post vaccination while the neutrophil percentage showed slight increased from the 2nd to 5th day then returned to normal level. The eosinophils (E), monocytes (M) and basophils (B) percentage remained within the normal limits.
On studying the effect of challenge infection in vaccinated group, it was found that there was no big difference in the mean total and differential leukocytic count, could be detected in vaccinated challenge calves while in case of artificial infection of non vaccinated calves with Adeno-virus, there was highly significant decrease in the mean total leukocytic counts from the 2nd to 7th days post infection accompanied with high significant decrease in lymphocytes percentage from the 2nd to 6th day while the mean neutrophils percentage showed significant increase from 3rd to 8th day post infection, and the monocytes percentage showed significant increase from 2nd to 5th days post infection, then there was moderate significant increase in total leukocytic count was detected on the 8th to 10th days post infection.
On conclusion, the prepared freeze dried inactivated Adeno-virus vaccine was safe, pure, potent and efficient; inducing protection against Adeno-virus infection, which attain 6 months immunity in susceptible calves. The vaccine should be used in national vaccination program in Egypt alone or in combination with other agents of pneumoenteritis syndromes (BVD, D3R, Pasteurella, .... etc.).