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Abstract This study was carried out in the Laboratory of the Molecular Biology Department at the Genetic Engineering and Biotechnology Research Institute, University of Sadat city, Egypt and the experimental farm, Cotton Research Institute, Sakha, Kafr El- Sheikh, Agricultural Research Center, Egypt during the period from 2010-2013. The present study included two experiments. The first one concerned with the study of 37 genotypes for morphological, yield and its components. The seconed experiment was carried out under different molecular markers to characterize the relationship between polymorphism and some quantitative traits. Using different ways of plant genotyping, protein, SSR, ISSR and RAPD markers to identify the relationship between morphological and yield characters and molecular markers. Mean performance for agronomic, yield and its components studied characters: For plant height (cm) the highest mean values were obtained from the genotype G.87 followed by Dandra and P.H.P. while the lowest value were obtained from Suven, G.89XPS6 and P62. For the position of the first fruiting node the highest mean values were obtained from genotypes G.86, G.88 and G.68. On the other hand, the lowest mean value were obtained from the genotype Menuofi followed by Karchenky and G.75x Sea. For the days to first flower the highest mean values were obtained from G.86 followed by Ashmouni and G.76 while the most desirable lowest mean values were obtained from the genotype Karchenky followed by G.93 and PS6. Regarding the number of fruiting branches per plant the most desirable highest mean values were obtained from genotype G.45, G.76 and Menuofi While the lowest mean values were obtained from G.89XG.86, G.74 and G.86. The highest mean values for the number of vegetative branches per plant were obtained from G.68, G.76 and G.71. While the lowest mean values were obtained from PS6, Suven and G.89XPS6. For the boll weight (B.W.) the highest mean values obtained from 10229XG.86, G89XG86 and G75XSea, while the lowest mean values were obtained from G.85, G.89 and G.76. Concerning the seed cotton yield per plant (S.C.Y./ P.) the highest mean values were obtained from genotypes C.B58, G.89 and Ashmouni, while the lowest mean values were obtained from G.77, Dandra and BBB. Regarding the lint yield per plant (L.Y. / P.) the highest mean values were obtained G.84 X (G.70XG.51B) X P62, C.B58 and G.89, while the lowest mean values were obtained from G.77, Dandra and BBB. For the lint percentage (L.%) the highest mean values were obtained from P.H.P, G.85 and 10229XG.86, while the lowest mean values were obtained from G.87, G.92 and G.88 . Concerning seed index (S.I), the highest mean values were obtained from Dandra, 1022XG.86 and Ashmouni, while the lowest mean values were obtained from G.76, G.87 and G.70. Regarding chlorophyll ratio the highest mean values were obtained from genotypes Ashmouni, G.85 and BBB, while the lowest mean values were obtained from genotypes G.70, G.76 and G.87. For the Fiber length (F.L.), the highest mean values were obtained from genotypes G.93, G.45 and G.70, while the lowest mean values were obtained from Suven, PS6 and Karchenky. Regarding the Fiber fineness (F.F.), the highest mean values were obtained from genotype Sea, Dandra and G.80, while the lowest mean values were obtained from G.71, G.45 and G.80XPS6. Concerning the fiber strength. (F.S.), the highest mean values were obtained from G.45, G.68 and G.92, while the lowest mean values were obtained from BBB, G80 and Sea. Total soluble protein analysis: The bands have Mw 110 KDa, 60KDa and 50KDa were present in genotypes which recorded the lowest mean values for plant height and were absent genotypes recorded the highest mean values for plant height, these bands can be considered as a positive marker for plant height. The bands with Mw 95 KDa and 55KDa were present in genotypes which recoreded the highest mean values for plant height and were absent in genotypes which recorded the lowest mean values for plant height these bands can be considered as negative biochemical marker for plant height. The band with Mw 77 KDa was present in genotypes which recorded the lowest mean values in (P.F.F.N) and was absent in genotypes which recorded the highest mean values in (P.F.F.N), this band can be considered as a positive marker for position of the first fruiting node. The bands with Mw 50 and 42 KDa were present in genotypes which recorded the highest mean values in boll weight (B.W.) and were absent in genotype which recorded the lowest mean values for boll weight, this two bands can be considered as positive markers for Boll weight. The band with Mw 35KDa was present in genotype which recorded the highest mean values in lint percentage (L. %), this band can be considered as positive markers for lint percentage (L. %). The band has Mw 70 KDa can be considered as a negative marker for chlorophyll content. The band with Mw 40KDa was present in genotype which recorded the highest mean values in lint cotton yield (L.Y/P), this band can be considered as positive markers for lint cotton yield (L.Y/P). Concerning the band has Mw 33 KDa can be considered as a negative markers for fiber length (F.L.). Regarding the band has Mw 55 KDa can be considered as a positive marker for fiber strength (F.S.). |